
-
抗体試薬
- フローサイトメトリー用試薬
-
ウェスタンブロッティング抗体試薬
- イムノアッセイ試薬
-
シングルセル試薬
- BD® AbSeq Assay
- BD Rhapsody™ Accessory Kits
- BD® OMICS-One Immune Profiler Protein Panel
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD® OMICS-One Protein Panels
-
細胞機能評価のための試薬
-
顕微鏡・イメージング用試薬
-
細胞調製・分離試薬
-
- BD® AbSeq Assay
- BD Rhapsody™ Accessory Kits
- BD® OMICS-One Immune Profiler Protein Panel
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD® OMICS-One Protein Panels
- Japan (Japanese)
-
Change country/language
Old Browser
Looks like you're visiting us from United States.
Would you like to stay on the current country site or be switched to your country?
BD Pharmingen™ Purified Hamster Anti-Mouse TCR β Chain
クローン H57-597 (RUO)

αβ TCR expression in spleen and thymus. C57BL/6 splenocytes were simultaneously stained with FITC-conjugated anti-mouse CD4 mAb RM4-5 (Cat. No. 553046/553047, left panels), FITC-conjugated anti-mouse CD8a mAb 53-6.7 (Cat. No. 553030/553031, left panels), and purified H57-597 (bottom left panel) monoclonal antibodies, followed by PE-conjugated anti-hamster IgG (Cat. No. 554056, left panels). C57BL/6 thymocytes were stained with purified mAb H57-597 (bottom right panel), followed by PE-conjugated anti-hamster IgG (Cat. No. 554056, right panels). Flow cytometry was performed on a BD FACScan™ flow cytometry system.


αβ TCR expression in spleen and thymus. C57BL/6 splenocytes were simultaneously stained with FITC-conjugated anti-mouse CD4 mAb RM4-5 (Cat. No. 553046/553047, left panels), FITC-conjugated anti-mouse CD8a mAb 53-6.7 (Cat. No. 553030/553031, left panels), and purified H57-597 (bottom left panel) monoclonal antibodies, followed by PE-conjugated anti-hamster IgG (Cat. No. 554056, left panels). C57BL/6 thymocytes were stained with purified mAb H57-597 (bottom right panel), followed by PE-conjugated anti-hamster IgG (Cat. No. 554056, right panels). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

αβ TCR expression in spleen and thymus. C57BL/6 splenocytes were simultaneously stained with FITC-conjugated anti-mouse CD4 mAb RM4-5 (Cat. No. 553046/553047, left panels), FITC-conjugated anti-mouse CD8a mAb 53-6.7 (Cat. No. 553030/553031, left panels), and purified H57-597 (bottom left panel) monoclonal antibodies, followed by PE-conjugated anti-hamster IgG (Cat. No. 554056, left panels). C57BL/6 thymocytes were stained with purified mAb H57-597 (bottom right panel), followed by PE-conjugated anti-hamster IgG (Cat. No. 554056, right panels). Flow cytometry was performed on a BD FACScan™ flow cytometry system.


BD Pharmingen™ Purified Hamster Anti-Mouse TCR β Chain

Regulatory Statusの凡例
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation and Storage
推奨アッセイ手順
It has been observed that pre-incubation of thymus cell suspensions at 37°C for 2 to 4 hours prior to staining enhances the ability of anti-CD3e and anti-TCR β chain mAbs to detect that T cell receptor on immature thymocytes.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
関連製品

.png?imwidth=320)


最近閲覧済み
The H57-597 antibody reacts with a common epitope of the β chain of the T-cell Receptor (TCR) complex on αβ TCR-expressing thymocytes, peripheral T lymphocytes, NK1.1+ thymocytes, and NK-T cells of all mouse strains tested. It does not react with γδ TCR-bearing T cells. In the fetal and adult thymus, the TCR β-chain may form homodimers or pair with the pre-TCR α-chain on the surface of immature thymocytes before TCR α-chain expression. Plate-bound or soluble H57-597 antibody activates αβ TCR-bearing T cells, and plate-bound mAb can induce apoptotic death.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (3)
-
Gascoigne NR. Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3. J Biol Chem. 1990; 265(16):9296-9301. (Clone-specific: Immunofluorescence, Immunoprecipitation). View Reference
-
Kruisbeek AM, Shevach EM. Proliferative assays for T cell function. Curr Protoc Immunol. 2004; 3:3.12.1-3.12.14. (Clone-specific: Activation, Functional assay, Stimulation). View Reference
-
Kubo RT, Born W, Kappler JW, Marrack P, Pigeon M. Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors. J Immunol. 1989; 142(8):2736-2742. (Immunogen: Activation, Flow cytometry, Functional assay, Stimulation). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.