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Flow cytometric analysis of MIP-1α (CCL3) expression on stimulated Rhesus macaque (Macaca mulatta) peripheral blood monocytes. Rhesus PBMC were stimulated with rhIFN-ɣ (10 ng/ml, 2 hours), then LPS (1 µg/ml, 2 hours) in the presence of GolgiStop™ (Cat. No. 554724; aka monensin 2 µM). The PBMC were harvested, stained with FITC Mouse Anti-Human CD14 antibody (Cat. No. 555397), fixed, permeabilized, and subsequently stained with APC Mouse Anti-Human MIP-1α (CCL3) (Cat. No. 551533). Two-color dot plot was derived from gated events with the forward and side-scattering characteristics of viable monocytes.
BD Pharmingen™ APC Mouse Anti-Human MIP-1α (CCL3)
Regulatory Statusの凡例
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation and Storage
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
The 11A3 monoclonal antibody specifically recognizes macrophage inflammatory protein-1α (MIP-1α), a member of the CC chemokine family of proteins. It is produced by T cells, B cells, Langerhans cells, neutrophils and macrophages. MIP-1α plays a role as an inhibitor of stem cell proliferation and as a chempattractant of B cells, eosinophils and cytotoxic T cells. Clone 11A3 also cross-reacts with an intracellular component of rhesus and cynomolgus macaque-LPS-stimulated peripheral blood monocytes. The reactivity pattern observed on CD14-positive cells is similar to that seen on normal human peripheral blood monocytes.
Development References (3)
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Rollins BJ. Chemokines. Blood. 1997; 90(3):909-928. (Biology). View Reference
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Vaddi K, Keller M, Newton RC. The chemokine factsbook. San Diego: Academic Press; 1997:205 p.
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Wolpe SD, Cerami A. Macrophage inflammatory proteins 1 and 2: members of a novel superfamily of cytokines. FASEB J. 1989; 3(14):2565-2573. (Biology). View Reference
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