FITC Mouse IgM, κ Isotype Control

BD Pharmingen™ FITC Mouse IgM, κ Isotype Control

Name: FITC Mouse IgM, κ Isotype Control
Brand: FITC Mouse IgM, κ Isotype Control
SKU: 551448

クローン G155-228 (RUO)

Flow cytometric analysis of FITC Mouse IgM, κ Isotype Control expression on peripheral blood lymphocytes of Rhesus macaque (macaca mulatta). Whole blood was stained with either FITC Mouse IgM, κ Isotype Control (Cat. No. 551448; solid line histogram) or left unstained (dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes.

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カタログ番号:555510

希望小売価格:¥25,300

単位:EA (1 Each)

容量:

50 Tests

数量:

見積に追加

見積リストに保存バルク試薬見積の依頼

製品詳細

Brand: BD Pharmingen™

Alternative Name: Anti-TNP

Reactivity: Rhesus, Cynomolgus, Baboon (QC Testing)

Isotype: Mouse BALB/c IgM, κ

Immunogen: TNP-Keyhole Limpet Hemocyanin

Application: Flow cytometry, Isotype control (Routinely Tested)

Vol. Per Test: 20 µl

RRID: AB_394198

Storage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

データシート

Technical Data Sheet (TDS)

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BD Pharmingen™ Purified Mouse Anti-Human CD54(HA58) RUO

カタログ番号 555510

551448 Rev. 4

抗体の詳細

G155-228

The G155-228 antibody is specific for the hapten trinitrophenol (TNP), a hapten not expressed on human, non-human primate, mouse, or rat primary cells or cell lines. In the absence of specific binding, this immunoglobulin may bind non-specifically to Fc receptors.

This reagent has been used as isotype control in immunophenotypic studies with baboon, rhesus, or cynomolgus peripheral blood cells.

551448 Rev. 4

フォーマットの詳細

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: FITC

励起源: Blue 488 nm

最大励起波長: 494 nm

最大蛍光波長: 518 nm

551448 Rev.4

引用＆参考文献

551448 Rev. 4

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

BD Pharmingen™ FITC Mouse IgM, κ Isotype Control

クローン G155-228 (RUO)

Preparation and Storage

Product Notices

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