Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Japan (Japanese)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      Purified Rat Anti-Human CD184
      Purified Rat Anti-Human CD184
      Detection of CXCR4 expression on human peripheral lymphocytes by purified 1D9. Human lysed whole blood were stained with 0.25 µg of purified 1D9 using 3-step staining protocol outlined below and anti-human CD4-FITC (Cat. No. 555346) The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using purified rat IgG2a (Cat. No. 555841) as isotype control. The quadrant markers for the bivariate dot plots were set based on the isotype control. The samples were analyzed with a FACScan Flow Cytometer (BD Biosciences, San Jose, CA).
      Purified Rat Anti-Human CD184
      Detection of CXCR4 expression on human peripheral lymphocytes by purified 1D9. Human lysed whole blood were stained with 0.25 µg of purified 1D9 using 3-step staining protocol outlined below and anti-human CD4-FITC (Cat. No. 555346) The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using purified rat IgG2a (Cat. No. 555841) as isotype control. The quadrant markers for the bivariate dot plots were set based on the isotype control. The samples were analyzed with a FACScan Flow Cytometer (BD Biosciences, San Jose, CA).
      製品詳細
      Down Arrow Up Arrow


      BD Pharmingen™
      CXCR4, Fusin
      Human (QC Testing)
      Rat IgG2a, κ
      Human CXCR4 fusion protein
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      AB_394191
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      推奨アッセイ手順

      The purified 1D9 antibody can be used for the immunofluorescent staining and flow cytometric analyses of human leukocytes and cell lines that express CXCR4 (see figure).

      A multistep step staining procedure is recommended to amplify immunofluorescent signals for the flow cytometric analysis of human CXCR4 expression:

      Step 1:   Incubate 10e6 cells with 0.06 - 0.5 µg of purified 1D9 antibody at 4°C for 15 - 20 minutes. Wash cells two times with staining medium containing sodium azide (e.g., Dulbecco's PBS or tissue culture medium [without phenol red and biotin] with 0.09% sodium azide and 2% heat-inactivated FCS or 0.2% BSA).

      Step 2:   Incubate the cells with biotinylated mouse anti-rat IgG2a (Cat. No. 553894) at 4°C for 20 minutes. Wash cells two times.

      Step 3:   Incubate the cells with ≤0.06 µg of streptavidin-phycoerythrin (Cat. No. 554061) at 4°C for 20 minutes. Wash two times. Resuspend cells in staining medium and analyze stained cells by flow cytometry.

      詳細を表示 blue down arrow 表示項目を減らす blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      詳細を表示 blue down arrow 表示項目を減らす blue up arrow
      551413 Rev. 1
      抗体の詳細
      Down Arrow Up Arrow
      1D9

      The monoclonal antibody 1D9 reacts with the human CD184 which is also known as CXC chemokine receptor, CXCR4. CXCR4 (previously known as fusin, LESTR and HUMSTR), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokines, SDF-1/CXCL12. CXCR4 was widely expressed by hematopoietic and non-hematopoietic cell types including PMN, monocytes, T cells, B cells, CD34+ progenitor cells, endothelial cells, neurons and astrocytes. The human CXCR4 is used by T-tropic HIV-1 as a co-receptor for viral entry. The human CXCR4 gene has been mapped to chromosome 2q21. The immunogen used to generate 1D9 hybridoma was human CXCR4 fusion protein.

      This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

      551413 Rev. 1
      フォーマットの詳細
      Down Arrow Up Arrow
      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      551413 Rev.1
      引用＆参考文献
      Down Arrow Up Arrow

      Development References (11)

      1. Bleul CC, Farzan M, Choe H, et al. The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry. Nature. 1996; 382(6594):829-833. (Biology). View Reference
      2. Bleul CC, Wu L, Hoxie JA, Springer TA, Mackay CR. The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes.. Proc Natl Acad Sci U S A. 1997; 94(5):1925-1930. (Biology). View Reference
      3. Federsppiel B, Melhado IG, Duncan AM, et al. Molecular cloning of the cDNA and chromosomal localization of the gene for a putative seven-transmembrane segment (7-TMS) receptor isolated from human spleen. Genomics. 1993; 16(3):707-712. (Biology). View Reference
      4. Feng Y, Broder CC, Kennedy PE, Berger EA. HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science. 1996; 272(5263):872-877. (Biology). View Reference
      5. Forster R, Kremmer E, Schubel A, et al. Intracellular and surface expression of the HIV-1 coreceptor CXCR4/fusin on various leukocyte subsets: rapid internalization and recycling upon activation. J Immunol. 1998; 160(3):1522-1531. (Immunogen). View Reference
      6. Gupta SK, Lysko PG, Pillarisetti K, Ohlstein E, Stadel JM. Chemokine receptors in human endothelial cells. Functional expression of CXCR4 and its transcriptional regulation by inflammatory cytokines. J Biol Chem. 1998; 273(7):4282-4287. (Biology). View Reference
      7. He J, Chen Y, Farzan M, et al. CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia. Nature. 1997; 385(6617):645-649. (Biology). View Reference
      8. Hesselgesser J, Halks-Miller M, DelVecchio V, et al. CD4-independent association between HIV-1 gp120 and CXCR4: functional chemokine receptors are expressed in human neurons. Curr Biol. 1997; 7(2):112-121. (Biology). View Reference
      9. Loetscher M, Geiser T, O'Reilly T, Zwahlen R, Baggiolini M, Moser B. Cloning of a human seven-transmembrane domain receptor, LESTR, that is highly expressed in leukocytes. J Biol Chem. 1994; 269(1):232-237. (Biology). View Reference
      10. Nomura H, Nielsen BW, Matsushima K. Molecular cloning of cDNAs encoding a LD78 receptor and putative leukocyte chemotactic peptide receptors. Int Immunol. 1993; 5(10):1239-1249. (Biology). View Reference
      11. Oberlin E, Amara A, Bachelerie F, et al. The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1. Nature. 1996; 382(6594):833-835. (Biology). View Reference
      すべて表示する (11) 表示項目を減らす
      551413 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback