BD Pharmingen™ Z-LEHD-FMK, Caspase-9 Inhibitor
Preparation and Storage
Z-LEHD-FMK, Caspase-9 Inhibitor is provided as a lyophilized powder. Store the lyophilized inhibitor at -20˚C. Reconstitute the inhibitor in DMSO before use. The reconstituted inhibitor may be stored in small aliquots at -20˚C.
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The Z-LEHD-FMK inhibitor can be used for both in vivo and in vitro cell based assays to measure the inhibition of apoptosis. While there are numerous methods to induce and measure apoptosis, Annexin V staining can be used on Jurkat cells (Human T-cell leukemia; ATCC TIB-152) for evaluating apoptosis (see figure).
Reconstitute 1.0 mg of Z-LEHD-FMK in DMSO. A 10 mM stock solution may be prepared by dissolving 1.0 mg of Z-LEHD-FMK in 124 µl DMSO. When using the Z-LEHD-FMK inhibitor, the optimal concentration needed may vary with the experimental system being used and investigators are highly encouraged to titrate the reagent. As a precautionary note, please do not exceed a final DMSO concentration of 0.2% as higher levels may increase the risk for cellular toxicity which may mask the effect of the caspase inhibitor.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.