PE Mouse Anti-Human ZAP-70

BD™ PE Mouse Anti-Human ZAP-70

Name: PE Mouse Anti-Human ZAP-70
Brand: BD™
SKU: 344635

クローン 1E7.2

(RUO (GMP))

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製品詳細

Brand: BD™

Reactivity: Human

Isotype: Mouse IgG1, κ

Immunogen: Human

Application: Flow cytometry

Concentration: 12.5 μg/mL

Vol. Per Test: 20 μL

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation and Storage

The FITC and PE conjugates are each supplied as 12.5 μg in 1.0 mL (12.5 μg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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抗体の詳細

1E7.2

Anti–ZAP-70, clone 1E7.2, is generated from the fusion of AG8.553 myeloma cells with spleen cells from BALB/c mice immunized with a ZAP-70 peptide 282-307 conjugated to KLH.

Anti–ZAP-70 is a 70-kilodalton (kd) protein kinase, a member of the Syk family of protein tyrosine kinases that is associated with the zeta chain of the CD3/TCR complex. Anti–ZAP-70 plays a crucial role in T-cell signal transduction and thymocyte development.

344635 Rev. 1

フォーマットの詳細

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: PE

励起源: Yellow-Green 488 nm, 532 nm, 561 nm

最大励起波長: 496 nm, 566 nm

最大蛍光波長: 576 nm

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引用＆参考文献

Development References (7)

Chan AC, Iwashima M, Turch CW, Weiss A. ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR ζ chain. Cell. 1992; 71:649-662. (Biology).
Chu DH, van Oers NS, Malissen M, Harris J, Elder M, Weiss A. Pre-T cell receptor signals are responsible for the down-regulation of Syk protein tyrosine kinase expression. J Immunol. 1999; 163(5):2610-2620. (Biology). View Reference
Crespo M, Bosch F, Villamor N, et al. ZAP-70 expression as a surrogate for immunoglobulin-variable-region mutations in chronic lymphocytic leukemia. N Engl J Med. 2003; 348:1764-1775. (Biology).
Orchard JA, Ibbotson RE, Davis Z, et al. ZAP-70 expression and prognosis in chronic lymphocytic leukaemia. Lancet. 2004; 363:105-111. (Biology).
Qian D, Lev S, van Oers NSC, Dikic I, Schlessinger J, Weiss A. Tyrosine phosphorylation of Pyk2 is selectively regulated by Fyn during TCR signaling. J Exp Med. 1997; 185:1253-1259. (Biology).
Rassenti LZ, Huynh L, Toy TL, et al. ZAP-70 compared with immunoglobulin heavy-chain gene mutation status as a predictor of disease progression in chronic lymphocytic leukemia. N Engl J Med. 2004; 351:893-901. (Biology).
Straus DB, Weiss A. The CD3 chains of the T cell antigen receptor associate with the ZAP-70 tyrosine kinase and are tyrosine phosphorylated after receptor stimulation. J Exp Med. 1993; 178:1523-1530. (Biology).

すべて表示する (7)

344635 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.