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Anti-Human CD5 FITC/CD10 PE/CD19 PerCP-Cy™5.5
製品詳細
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BD Oncomark™
Human
Flow cytometry
体外診断用医薬品
Phosphate-buffered saline (PBS) containing gelatin and 0.1% sodium azide.


説明

The CD5 antibody, clone L17F12, is derived from hybridization of NS-1/Ag4 mouse myeloma cells with spleen cells from BALB/c mice immunized with human T-ALL cells.

The CD10 antibody, clone HI10a, is derived from the hybridization of P3-63-Ag8.653 mouse myeloma cells with spleen cells of BALB/c mice immunized with blasts from a patient with acute CALLA leukemia.

The CD19 antibody, clone SJ25C1, is derived from hybridization of Sp2/0 mouse cells with spleen cells from BALB/c mice immunized with NALM1 + NALM16 cells.

The CD5 antibody recognizes a human T-lymphocyte antigen, with a molecular weight of 67 kilodaltons (kDa).

The CD10 antibody (Anti-CALLA) recognizes a human common acute lymphoblastic leukemia antigen (CALLA), with a molecular weight of 100 kDa. The CD10 antigen is identical to human membrane-associated neutral endopeptidase (NEP; EC 3.3.24.11), also known as enkephalinase.

The CD19 antibody (SJ25C1) recognizes a 90-kDa antigen that is present on human B lymphocytes.

調製と保管

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

341138 Rev. 1
構成品
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説明 クローン アイソタイプ EntrezGene ID
CD5 L17F12 IgG2a, κ 921
CD19 PerCP-Cy5.5 SJ25C1 IgG1, κ 930
CD10 N/A N/A N/A
341138 Rev. 1
引用&参考文献
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開発者向け参考資料 (33)

  1. Almasri NM, Iturraspe JA, Braylan RC. CD10 expression in follicular lymphoma and large cell lymphoma is different from that of reactive lymph node follicles. Arch Pathol Lab Med. 1998; 122:539-544. (Biology).
  2. Baldini L, Cro L, Cortelezzi A, et al. Immunophenotypes in "classical" B-cell chronic lymphocytic leukemia. Correlation with normal cellular counterpart and clinical findings. Cancer. 1990; 66(8):1738-1742. (Biology).
  3. Cabezudo E, Carrara P, Morilla R, Matutes E. Quantitative analysis of CD79b, CD5 and CD19 in mature B-cell lymphoproliferative disorders. Haematologica. 1999; 84(5):413-418. (Biology).
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  7. DiGiuseppe JA, Borowitz MJ. Clinical utility of flow cytometry in the chronic lymphoid leukemias. Semin Immunol. 1998; 25(1):6-10. (Biology). 参考文献を見る
  8. Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R. Studies of a human T lymphocyte antigen recognized by a monoclonal antibody.. Proc Natl Acad Sci USA. 1981; 78(3):1791-5. (Biology). 参考文献を見る
  9. Freedman AS, Nadler LM. Immunologic markers in non-Hodgkin's lymphoma. Hematol Oncol Clin North Am. 1991; 5(5):871-889. (Biology).
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  11. Geisler CH, Larsen JK, E HN, et al. Prognostic importance of flow cytometric immunophenotyping of 540 consecutive patients with B-cell chronic lymphocytic leukemia. Blood. 1991; 78:1795-1802. (Biology).
  12. Greaves M. In:Knapp W, ed. Leukemia Markers. New York: Academic Press; 1981:19.
  13. Hoffkes HG, Schmidtke G, Uppenkamp M, Schmucker U. Multiparametric immunophenotyping of B cells in peripheral blood of healthy adults by flow cytometry. Clin Diagn Lab Immunol. 1996; 3:30-36. (Biology).
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  16. Knowles RW. Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II. Human T Lymphocytes. New York, NY: Springer-Verlag; 1986:259-288.
  17. LeBien TW, McCormack RT. The common acute lymphoblastic leukemia antigen (CD10)--emancipation from a functional enigma.. Blood. 1989; 73(3):625-35. (Biology). 参考文献を見る
  18. Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology). 参考文献を見る
  19. Ledbetter JA, Frankel AE, Herzenberg. Human Leu T-cell differentiation antigens: quantitative expression on normal lymphoid cells and cell lines. In: Hammerling G, Hammerling U, Kearney J, ed. Monoclonal Antibodies and T Cell Hybridomas: Perspectives and Technical News. New York: Elsevier/North Holland Biomedical Press; 1981:16-22.
  20. Letarte M, Vera S, Tran R, et al. Common acute lymphocytic leukemia antigen is identical to neutral endopeptidase. J Exp Med. 1988; 168(4):1247-1253. (Biology). 参考文献を見る
  21. Loken MR, Shah VO, Dattilio KL, Civin CI. Flow cytometric analysis of human bone marrow. II. Normal B lymphocyte development. Blood. 1987; 70(5):1316-1324. (Biology). 参考文献を見る
  22. Moldenhauer G, Dörken B, Schwartz R, Pezzutto A, Knops J, Hammerling GJ. Analysis of ten B lymphocyte-specific workshop monoclonal antibodies. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York: Springer-Verlag; 1986:61-67.
  23. Nadler LM. B Cell/Leukemia Panel Workshop: summary and comments. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York: Springer-Verlag; 1986:3-43.
  24. Pui CH, Rivera GK, Hancock ML, et al. Clinical significance of CD10 expression in childhood acute lymphoblastic leukemia. Leukemia. 1993; 7(1):35-40. (Biology).
  25. Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). 参考文献を見る
  26. Royston I, Majda JA, Baird SM, Meserve BL, Griffiths JC. Human T-cell antigens defined by monoclonal antibodies: The 65,000-dalton antigen of T cells (T65) is also found on chronic lymphocytic leukemia cells bearing surface immunoglobulin. J Immunol. 1980; 125:725. (Biology).
  27. Schmidt CJ, Domenico L, Ward P, Barcos MP, Stewart CC. Aberrant antigen expression detected by multiparameter three color flow cytometry in intermediate and high grade B-cell lymphomas. Leuk Lymphoma. 1999; 34:539-544. (Biology).
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  31. Warnke RA, Levy R. Detection of T and B cell antigens with hybridoma monoclonal antibodies: a biotinavidin-horseradish peroxidase method. J Histochem Cytochem. 1980; 28:771-776. (Biology).
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  33. Zola H. CD10 Workshop Panel report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:505-507.
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341138 Rev. 1

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