Anti-Human Lineage Cocktail 1 (lin 1) (CD3, CD14, CD16, CD19, CD20, CD56)

BD FastImmune™ Anti-Human Lineage Cocktail 1 (lin 1) (CD3, CD14, CD16, CD19, CD20, CD56)

Name: Anti-Human Lineage Cocktail 1 (lin 1) (CD3, CD14, CD16, CD19, CD20, CD56)
Brand: Anti-Human Lineage Cocktail 1 (lin 1) (CD3, CD14, CD16, CD19, CD20, CD56)
SKU: 340546

Protocol Library

Scientific Resources

Protocol Library

Scientific Resources

カタログ番号:340546

希望小売価格:¥120,000

単位:EA (1 Each)

容量:

50 Tests

数量:

見積に追加

見積リストに保存

製品詳細

Brand: BD FastImmune™

Concentration: 140.6 μg/mL

Reactivity: Human

Application: Flow cytometry , Intracellular staining (flow cytometry), Northern blot

Regulatory Status: RUO (GMP)

RRID: AB_400053

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

説明

The following are each composed of mouse IgG₁ heavy chains and kappa light chains:

CD3, clone SK7

CD16, clone 3G8

CD19, clone SJ25C1

CD20, clone L27

The following are each composed of mouse IgG_2b heavy chains and kappa light chains:

CD14, clone MφP9

CD56, clone NCAM16.2

The Lineage Cocktail 1 (lin 1) contains antibody clones that, in combination, stain lymphocytes, monocytes, eosinophils, and neutrophils. It includes CD3, CD14, CD16, CD19, CD20, and CD56. Peripheral blood dendritic cells and basophils can be distinguished from other leucocytes by their lack of staining with lin 1.

調製と保管

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

製品通知

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

データシート

テクニカルデータシート（TDS）

340546 Rev. 1

構成品

説明	クローン	アイソタイプ	EntrezGene ID
CD3 FITC	SK7	IgG1, κ	N/A
CD14 FITC	MΦP9	IgG2b, κ	N/A
CD16 FITC	3G8	IgG1, κ	N/A
FITC Mouse anti-Human CD19	SJ25C1	IgG1, κ	930
CD20 FITC	L27	IgG1, κ	N/A
CD56 FITC	NCAM16.2	IgG2b, κ	N/A

340546 Rev. 1

引用＆参考文献

開発者向け参考資料 (14)

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Clinical and Laboratory Standards Institute. 2005. (Biology).
Freudenthal PS, Steinman RM. The distinct surface of human blood dendritic cells, as observed after an improved isolation method. Proc Natl Acad Sci USA. 1990; 87:7698-7702. (Biology).
Grouard G, Durand I, Filgueira L, Banchereau J, Liu YJ. Dendritic cells capable of stimulating T cells in germinal centres.. Nature. 1996; 384(6607):364-7. (Biology). 参考文献を見る
Markowicz S, Engleman EG. Granulocyte-macrophage colony-stimulating factor promotes differentiation and survival of human peripheral blood dendritic cells in vitro. J Clin Invest. 1990; 85:955-961. (Biology).
Mueller R, Heusser CH, Rihs S, Brunner T, Bullock GR, Dahinden CA. Immunolocalization of intracellular interleukin-4 in normal human peripheral blood basophils. Eur J Immunol. 1994; 24:2935-2940. (Biology).
O'Doherty U, Peng M, Gezelter S, et al. Human blood contains two subsets of dendritic cells, one immunologically mature and the other immature.. Immunology. 1994; 82(3):487-93. (Biology). 参考文献を見る
Olweus J, BitMansour A, Warnke R, et al. Dendritic cell ontogeny: a human dendritic cell lineage of myeloid origin. Proc Natl Acad Sci USA. 1997; 94:12551-12556. (Biology).
Stain C, Stockinger H, Scharf M, et al. Human blood basophils display a unique phenotype including activation linked membrane structures. Blood. 1987; 70:1872-1879. (Biology).
Steinman R M. The dendritic cell system and its role in immunogenicity. Annu Rev Immunol. 1991; 9:271-296. (Biology).
Thomas R, Lipsky PE. Human peripheral blood dendritic cell subsets. Isolation and characterization of precursor and mature antigen-presenting cells.. J Immunol. 1994; 153(9):4016-28. (Biology). 参考文献を見る
Valent P, Besemer J, Muhm M, Majdic O, Lechner K, Bettelheim P. Interleukin 3 activates human blood basophils via high-affinity binding sites. Proc Natl Acad Sci USA. 1989; 86:5542-5546. (Biology).
Valent P. Immunophenotypic characterization of human basophils and mast cells. Chem Immunol. 1995; 61:34-48. (Biology).

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340546 Rev. 1

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