Skip to main content Skip to navigation
Anti-Human CD4 FITC/CD69 PE/CD3 PerCP

BD FastImmune™ Anti-Human CD4 FITC/CD69 PE/CD3 PerCP

563906
Inquire
EA (1 Each)
50 Tests
製品詳細
Down Arrow


BD FastImmune™
Human
Flow cytometry
RUO (GMP)
AB_400415
Phosphate buffered saline with BSA and 0.1% sodium azide.


説明

CD4, clone SK3, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes.

CD69, clone L78, is derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with lymph node cells from BALB/c mice immunized with a CD8+ alloantigen-directed cytotoxic T-lymphocyte (CTL) cell line.

CD3, clone SK7, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes.

CD4 recognizes an antigen that interacts with class II molecules of the major histocompatibility complex (MHC). CD4 is the primary receptor for the human immunodeficiency virus (HIV). The cytoplasmic portion of the antigen is associated with the protein tyrosine kinase p56lck. The CD4 antigen may regulate the function of theCD3 antigen/T-cell antigen receptor (TCR) complex. CD4 also reacts with monocytes/macrophages that have an antigen density lower than that on helper/inducer T lymphocytes.  CD69 recognizes a very early human lymphocyte activation antigen.

The CD69 antigen is a surface homodimer formed by the association of 28-kilodalton (kd) and 32-kd chains that are held together by disulfide bridges.

CD3 recognizes the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kd. The antigen recognized by the CD3 antibody is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kd).

調製と保管

The FastImmune reagent is supplied as a combination of CD4 FITC, CD69 PE, and CD3 PerCP in 1.0 mL of phosphate-buffered saline (PBS) containing bovine serum albumin (BSA) and 0.1% sodium azide. The vial should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340365 Rev. 1
構成品
Down Arrow
説明 クローン アイソタイプ EntrezGene ID
N/A N/A | IgG1, κ,IgG1, κ,IgG1, κ N/A
340365 Rev. 1
引用&参考文献
Down Arrow

開発者向け参考資料 (8)

  1. Bernard A, Boumsell L, Hill C. Joint report of the first international workshop on human leucocyte differentiation antigens by the investigators of the participating laboratories: T2 protocol. In: Bernard A. A. Bernard .. et al., ed. Leucocyte typing : human leucocyte differentiation antigens detected by monoclonal antibodies : specification, classification, nomenclature = Typage leucocytaire : antigènes de différenciation leucocytaire humains révélés par les anticorps monoclonaux : "Rapports des études communes". Berlin New York: Springer-Verlag; 1984:25-60.
  2. Dalgleish AG, Beverley PC, Clapham PR, Crawford DH, Greaves MF, Weiss RA. The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus.. Nature. 312(5996):763-7. (Biology). 参考文献を見る
  3. Evans RL, Wall DW, Platsoucas CD, et al. Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen. Proc Natl Acad Sci U S A. 1981; 78(1):544-548. (Biology). 参考文献を見る
  4. Graber M, Bockenstedt LK, Weiss A. Signaling via the inositol phospholipid pathway by T cell antigen receptor is limited by receptor number.. J Immunol. 1991; 146(9):2935-43. (Biology). 参考文献を見る
  5. Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:3-30.
  6. Knowles RW. Immunochemical analysis of the T-cell–specific antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:259-288.
  7. Lanier LL, Allison JP, Phillips JH. Correlation of cell surface antigen expression on human thymocytes by multi-color flow cytometric analysis: implications for differentiation. J Immunol. 1986; 137(8):2501-2507. (Biology). 参考文献を見る
  8. Lanier LL, Buck DW, Rhodes L, et al. Interleukin 2 activation of natural killer cells rapidly induces the expression and phosphorylation of the Leu-23 activation antigen.. J Exp Med. 1988; 167(5):1572-85. (Biology). 参考文献を見る
すべて表示する (8) 表示項目を減らす
340365 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

 

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.