Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Japan (Japanese)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      Anti-Human FMC7 FITC/CD23 PE/CD5 PerCP-Cy™5.5/CD19 PE-Cy™7/CD38 APC/CD45 APC-Cy™7

      BD Oncomark™ Anti-Human FMC7 FITC/CD23 PE/CD5 PerCP-Cy™5.5/CD19 PE-Cy™7/CD38 APC/CD45 APC-Cy™7

      (RUO (GMP))
      製品詳細
      Down Arrow Up Arrow


      BD Oncomark™
      Human
      Flow cytometry
      RUO (GMP)
      Phosphate buffered saline with gelatin and 0.1% sodium azide.


      説明

      Clone FMC7 is generated from the fusion of mouse P3-NS1-1-AG4-1 myeloma cells with spleen cells from BALB/c mice immunized with human B-lymphoblastoid cell line HRIK.

      CD23, clone EBVCS-5, is derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with in vitro–transformed EBV cell line.

      CD5, clone L17F12, is derived from hybridization of mouse NS-1/Ag4 myeloma cells with spleen cells from BALB/c mice immunized with human T-acute lymphoblastic leukemia (ALL) cells.

      CD19, clone SJ25C1, is derived from the hybridization of mouse Sp2/0 cells with spleen cells from BALB/c mice immunized with NALM1 + NALM16 cells.

      CD38, clone HB7, is derived from hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with the BJAB cell line.

      CD45, clone 2D1, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood mononuclear cells (PBMCs).

      FMC7 recognizes a 105-kilodalton (kd) membrane glycoprotein expressed on a subset of B lymphocytes.

      CD23 recognizes a human B-lymphocyte differentiation antigen, Mr 45 kd, that is the low-affinity Fc epsilon receptor.

      CD5 recognizes a human T-lymphocyte antigen, Mr 67 kd.

      CD19 (SJ25C1) recognizes a 90-kd antigen that is present on human B lymphocytes.

      CD38 recognizes an integral membrane glycoprotein, Mr 45 kd, with a protein core of 35 kd.

      CD45 (Anti–HLe-1) recognizes human leucocyte antigens, Mr 180 to 220 kd, that are members of the T200 family.

      調製と保管

      Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

      詳細を表示 blue down arrow 表示項目を減らす blue up arrow
      338430 Rev. 1
      構成品
      Down Arrow Up Arrow
      説明 クローン アイソタイプ EntrezGene ID
      FMC7 FITC FMC7 IgM, N/A
      CD23 PE EBVCS-5 IgG1, κ N/A
      CD5 PerCP-Cy5.5 L17F12 IgG2a, κ N/A
      Human CD19 SJ25C1 IgG1, κ 930
      CD38 APC HB7 IgG1, κ N/A
      Human CD45 2D1 IgG1, κ N/A
      338430 Rev. 1
      引用＆参考文献
      Down Arrow Up Arrow

      開発者向け参考資料 (47)

      1. Bloem AC, Chand MA, Dollekamp I, Rijkers GT. Functional properties of human B cell subpopulations defined by monoclonal antibodies HB4 and FMC7. J Immunol. 1988; 140:768-773. (Biology).
      2. Brooks DA, Beckman IG, Bradley J, McNamara PJ, Thomas ME, Zola H. Human lymphocyte markers defined by antibodies derived from somatic cell hybrids, IV, A monoclonal antibody reacting specifically with a subpopulation of human B lymphocytes. J Immunol. 1981; 126:1373-1377. (Biology).
      3. Capron M, Jouault T, Prin L, et al. Functional study of a monoclonal antibody to IgE Fc receptor (Fc epsilon R2) of eosinophils, platelets, and macrophages.. J Exp Med. 1986; 164(1):72-89. (Biology). 参考文献を見る
      4. Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988; 37:377-388. (Biology).
      5. Clinical and Laboratory Standards Institute. 2005. (Biology).
      6. Deaglio S, Morra M, Mallone R, et al. Human CD38 (ADP-ribosyl cyclase) is a counter-receptor of CD31, an Ig superfamily member. J Immunol. 1998; 160(1):395-402. (Biology). 参考文献を見る
      7. Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:34-36.
      8. Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R. Studies of a human T lymphocyte antigen recognized by a monoclonal antibody.. Proc Natl Acad Sci USA. 1981; 78(3):1791-5. (Biology). 参考文献を見る
      9. Gadol N, Ault KA. Phenotypic and functional characterization of human Leu-1 (CD5) B cells. J Histochem Cytochem. 1986; 93:23. (Biology).
      10. Ghia P, Guida G, Stella S, et al. The pattern of CD38 expression defines a distinct subset of chronic lymphocytic leukemia (CLL) patients at risk of disease progression. Blood. 2003; 101(4):1262-1269. (Biology). 参考文献を見る
      11. Giorgi JV. Lymphocyte subset measurements: significance in clinical medicine. In: Rose NR, Friedman H, Fahey JL, ed. Manual of Clinical Laboratory Immunology. 3rd ed.. Washington, DC: American Society for Microbiology; 1986:236-246.
      12. Gordon J, Rowe M, Walker L, Guy G. Ligation of the CD23,p45 (Blast-2, EBVCS) antigen triggers the cell-cycle progression of activated B lymphocytes. Eur J Immunol. 1986; 16:1075-1080. (Biology).
      13. Gordon J, Webb A, Walker L, Guy G, Rowe M. Evidence of an association between CD23 and the receptor for a low molecular weight B-cell growth factor. Eur J Immunol. 1986; 16:1627. (Biology).
      14. Hassan IB, Hagberg H, Sundström C. Immunophenotype of hairy-cell leukemia. Eur J Haematol. 1990; 45:172-176. (Biology).
      15. Huh YO, Pugh WC, Kantarjian HM, et al. Detection of subgroups of chronic B-cell leukemias by FMC7 monoclonal antibody. Am J Clin Pathol. 1994; 101:283-289. (Biology).
      16. Jackson A. Basic phenotyping of lymphocytes: selection and testing of reagents and interpretation of data. Clin Immunol Newslett. 1990; 10:43-55. (Biology).
      17. Kikutani H, Inui S, Sato R, et al. Molecular structure of human lymphocyte receptor for immunoglobulin E.. Cell. 1986; 47(5):657-65. (Biology). 参考文献を見る
      18. Kilo MN, Dorfman DM. The utility of flow cytometric immunophenotypic analysis in the distinction of small lymphocytic lymphoma/chronic lymphocytic leukemia from mantle cell lymphoma. Am J Clin Pathol . 1996; 105:451-457. (Biology).
      19. Kintner C, Sugden B. Identification of antigenic determinants unique to the surfaces of cells transformed by Epstein-Barr Virus. Nature. 1981; 294:458-460. (Biology).
      20. Knowles RW. Immunochemical analysis of the T-cell–specific antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:259-288.
      21. Landay A, Ohlsson-Wilhelm B, Giorgi JV. Application of flow cytometry to the study of HIV infection. AIDS. 1990; 4(6):479-497. (Biology). 参考文献を見る
      22. Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology). 参考文献を見る
      23. Ledbetter JA, Frankel AE, Herzenberg. Human Leu T-cell differentiation antigens: quantitative expression on normal lymphoid cells and cell lines. In: Hammerling G, Hammerling U, Kearney J, ed. Monoclonal Antibodies and T Cell Hybridomas: Perspectives and Technical News. New York: Elsevier/North Holland Biomedical Press; 1981:16-22.
      24. Loken MR, Shah VO, Dattilio KL, Civin CI. Flow cytometric analysis of human bone marrow. II. Normal B lymphocyte development. Blood. 1987; 70(5):1316-1324. (Biology). 参考文献を見る
      25. Matutes E, Morilla R, Owusu-Ankomah K, Houliham A, Meeus P, Catovsky D. The immunophenotype of hairy cell leukemia (HCL). Proposal for a scoring system to distinguish HCL from B-cell disorders with hairy or villous lymphocytes. Leuk Lymphoma. 1994; 14:57-61. (Biology).
      26. Moldenhauer G, Dörken B, Schwartz R, Pezzutto A, Knops J, Hammerling GJ. Analysis of ten B lymphocyte-specific workshop monoclonal antibodies. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York: Springer-Verlag; 1986:61-67.
      27. Nadler LM. B Cell/Leukemia Panel Workshop: summary and comments. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York: Springer-Verlag; 1986:3-43.
      28. Nadler LM. Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York, NY: Springer-Verlag; 1986:25-26.
      29. Nicholson JKA, Jones BM. Immunophenotyping by flow cytometry: its use in HIV infection. Labmedica. 1989; 6:21-26. (Biology).
      30. Pezzutto A, Behm F, Callard RE. Flow cytometry analysis of the B-cell blind panel: joint report. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:165-174.
      31. Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). 参考文献を見る
      32. Reinherz EL, Kung PC, Goldstein G, Levey RH, Schlossman SF. Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage. Proc Natl Acad Sci U S A. 1980; 77(3):1588-1592. (Biology). 参考文献を見る
      33. Royston I, Majda JA, Baird SM, Meserve BL, Griffiths JC. Human T-cell antigens defined by monoclonal antibodies: The 65,000-dalton antigen of T cells (T65) is also found on chronic lymphocytic leukemia cells bearing surface immunoglobulin. J Immunol. 1980; 125:725. (Biology).
      34. Salazar-Gonzalez JF, Moody DJ, Giorgi JV, Martinez-Maza O, Mitsuyasu RT, Fahey JL. Reduced ecto-5'-nucleotidase activity and enhanced OKT10 and HLA-DR expression on CD8 (T suppressor/cytotoxic) lymphocytes in the acquired immune deficiency syndrome: evidence of CD8 cell immaturity. J Immunol. 1985; 135(3):1778-1785. (Biology). 参考文献を見る
      35. Schwinzer R. Cluster Report: CD45/CD45R. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:628-634.
      36. Tedder TF, Clement LT, Cooper MD. Discontinuous expression of a membrane antigen (HB-7) during B lymphocyte differentiation. Tissue Antigens. 1984; 24(3):140-149. (Biology). 参考文献を見る
      37. Tedder TF, Crain MJ, Kubagawa H, Clement LT, Cooper MD. Evaluation of lymphocyte differentiation in primary and secondary immunodeficiency diseases. J Immunol. 1985; 135(3):1785-1791. (Biology). 参考文献を見る
      38. Tedder TF, Zhou LJ, Engel P. The CD19/CD21 signal transduction complex of B lymphocytes. Immunol Today. 1994; 15(9):437-442. (Biology). 参考文献を見る
      39. Terstappen LW, Hollander Z, Meiners H, Loken MR. Quantitative comparison of myeloid antigens on five lineages of mature peripheral blood cells. J Leukoc Biol. 1990; 48(2):138-148. (Biology). 参考文献を見る
      40. Terstappen LW, Huang S, Picker LJ. Flow cytometric assessment of human T-cell differentiation in thymus and bone marrow. Blood. 1992; 79(3):666-677. (Biology). 参考文献を見る
      41. Terstappen LW, Huang S, Safford M, Lansdorp PM, Loken MR. Sequential generations of hematopoietic colonies derived from single nonlineage-committed CD34+ CD38– progenitor cells. Blood. 1991; 77:1218-1227. (Biology).
      42. Thorley-Lawson D, Nadler L, Bhan A, Schooley R. Blast-2 (EBVCS), an early cell surface marker of human B cell activation, is superinduced by Epstein Barr Virus. J Immunol. 1985; 134:3007-3012. (Biology).
      43. Warnke R, Miller R, Grogan T, Pederson M, Dilley J, Levy R. Immunologic phenotype in 30 patients with diffuse large-cell lymphoma.. N Engl J Med. 1980; 303(6):293-300. (Biology). 参考文献を見る
      44. Warnke RA, Levy R. Detection of T and B cell antigens with hybridoma monoclonal antibodies: a biotinavidin-horseradish peroxidase method. J Histochem Cytochem. 1980; 28:771-776. (Biology).
      45. Yukawa K, Kikutani H, Owaki H, et al. A B-cell– specific differentiation antigen, CD23, is a receptor for IgE (FcεR) on lymphocytes. J Immunol. 1987; 138:2576-2580. (Biology).
      46. Zipf RF, Fox R, Dilley J, Levy R. Definition of the high risk ALL patient by immunologic phenotyping withmonoclonal antibodies. Cancer Res. 1981; 41:4786. (Biology).
      47. Zola H, Neoh SH, Potter A, Melo JV, De Oliveria MSP, Catovsky D. Markers of differentiated B cell leukaemia: CD22 antibodies and FMC7 react with different molecules. Disease Markers. 1987; 5:227-235. (Biology).
      すべて表示する (47) 表示項目を減らす
      338430 Rev. 1

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

      Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

      Website Feedback