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BD Pharmingen™ Recombinant Human IL-5
(RUO)Recombinant Human IL-5
Regulatory Statusの凡例
Becton, Dickinson and Companyの書面による明示的な許諾を得た使用以外での製品の使用は固く禁じられています。
説明
Interleukin-5 (IL-5) is the lymphokine responsible for the activities attributed to eosinophil differentiating factor (EDF), B cell growth factor II (BCGFII), and T cell-replacing factor (TRF). IL-5 induces eosinophil differentiation and promotes eosinophil survival and activation. Recombinant human IL-5 is a 28 - 31 kD protein dimer as determined by SDS-PAGE (non-reducing conditions). Recombinant human IL-5 (Cat. No. 554606) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, containing bovine serum albumin, with no preservatives. Recombinant human IL-5 is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 0.1 ng per µg of human IL-5, as measured in chromogenic LAL assay.
調製と保管タイトルテキスト
推奨アッセイ手順
Upon initial thawing, recombinant human IL-5 (Cat. No. 554606) should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/mL carrier protein, such as human or bovine serum albumin, aliquoted and stored at -80°C. For in vitro biological assay use, carrier-protein concentrations of
0.5 - 1.0 mg/mL are recommended. For use as an ELISA standard, carrier-protein concentrations of 5 - 10 mg/mL are recommended. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. The product should not be diluted to less than 1 μg/mL for long term storage. Carrier proteins should be pre-screened for possible effects in each investigator's experimental system. Carrier proteins may have an undesired influence on experimental results due to toxicity, high endotoxin levels or possible blocking activity.
ELISA Standard: Recombinant human IL-5 (Cat. No 554606) can be useful as a quantitative standard for measuring human IL-5 protein levels using sandwich ELISA with purified JES1-39D10 (Cat. No. 554488) as a capture antibody and biotinylated JES1-5A10 (Cat. No.554491) as the detection antibody. To obtain linear standard curves, investigators may want to consider using doubling dilutions of recombinant human IL-5 from 2,000 - 15 pg/mL to be included in each ELISA plate. For measuring human IL-5 in serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Human IL-5 ELISA Set (Cat. No. 555202).
Bioassay: Investigators are advised that the Bioassay application is not routinely tested for this material and are highly encouraged to both titrate this material and include appropriate controls in relevant experiments. An activity range of 0.25 - 1.0 x 10^8 units/mg, encompassing an
ED50 = 100 - 400 pg/mL, has previously been reported using TF-1 as indicator cells for proliferation, with a unit defined as the amount of material needed to stimulate a half-maximal response at cytokine saturation.
Blocking: Recombinant human IL-5 (Cat. No 554606) can be used as a blocking control for flow cytometric analysis when used with fluorochrome-conjugated antibodies, such as PE-conjugated JES1-39D10 (Cat. No. 559332). Investigators are advised that the blocking application is not routinely tested for this material. Intracellular cytokine staining techniques and the use of blocking controls are described in detail by C.Prussin and D.Metcalfe.
製品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
コンパニオン製品
Development References (3)
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Kitamura T, Takaku F, Miyajima A. IL-1 up-regulates the expression of cytokine receptors on a factor-dependent human hemopoietic cell line, TF-1. Int Immunol. 1991; 3(6):571-577. (Methodology: Bioassay). View Reference
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Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: IC/FCM Block). View Reference
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Sideras P, Noma T, Honjo T. Structure and function of interleukin 4 and 5. Immunol Rev. 1988; 102:189-212. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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