FITC Mouse Anti-Rat CD4

BD Pharmingen™ FITC Mouse Anti-Rat CD4

Name: FITC Mouse Anti-Rat CD4
Brand: FITC Mouse Anti-Rat CD4
SKU: 554837

クローン OX-35 (RUO)

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Two-color analysis of the expression of CD4 on rat splenic leukocytes. Lewis splenocytes were simultaneously stained with FITC-conjugated anti-rat CD4 mAb clone OX-35 and PE-conjugated anti-rat CD3 mAb clone G4.18 (Cat. No. 554833). The CD3-negative CD4-dim cells are the monocyte/macrophage population. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

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カタログ番号:554477

希望小売価格:¥48,070

単位:EA (1 Each)

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製品詳細

Brand: BD Pharmingen™

Reactivity: Rat (QC Testing)

Isotype: Mouse BALB/c IgG2a, κ

Immunogen: Rat T-cell blasts

Application: Flow cytometry (Routinely Tested)

Concentration: 0.5 mg/ml

RRID: AB_395547

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

データシート

Technical Data Sheet (TDS)

関連製品

FITC Mouse IgG2a, κ Isotype Control RUO

サイズ 0.25 mg カタログ番号 553456

PE Mouse Anti-Rat CD3 RUO

サイズ 0.2 mg カタログ番号 554833

554837 Rev. 12

抗体の詳細

OX-35

The OX-35 clone recognizes the CD4 antigen on most thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), monocytes, macrophages, some dendritic cells, and microglia. CD4 is an antigen coreceptor on the T-cell surface that interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through it's association with the T-cell receptor complex and protein tyrosine kinase Lck. The OX-35 clone has been reported to bind to a different epitope of CD4 than that recognized by the W3/25 and OX-38 clones.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

554837 Rev. 12

フォーマットの詳細

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: FITC

最大励起波長: 494 nm

最大蛍光波長: 518 nm

554837 Rev.12

引用＆参考文献

View Development References

Development References (7)

Bañuls MP, Alvarez A, Ferrero I, Zapata A, Ardavin C. Cell-surface marker analysis of rat thymic dendritic cells. Immunology. 1993; 79(2):298-304. (Biology). View Reference
Bierer BE, Sleckman BP, Ratnofsky SE, Burakoff SJ. The biologic roles of CD2, CD4, and CD8 in T-cell activation. Annu Rev Immunol. 1989; 7:579-599. (Biology). View Reference
Ford AL, Foulcher E, Goodsall AL, Sedgwick JD. Tissue digestion with dispase substantially reduces lymphocyte and macrophage cell-surface antigen expression. J Immunol Methods. 1996; 194(1):71-75. (Biology: Depletion). View Reference
Janeway CA Jr. The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation. Annu Rev Immunol. 1992; 10:645-674. (Biology). View Reference
Jefferies WA, Green JR, Williams AF. Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages. J Exp Med. 1985; 162(1):117-127. (Immunogen: Flow cytometry, Functional assay, Immunoaffinity chromatography, Immunoprecipitation, Inhibition). View Reference
Liu L, Zhang M, Jenkins C, MacPherson GG. Dendritic cell heterogeneity in vivo: two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression. J Immunol. 1998; 161(3):1146-1155. (Biology). View Reference
Wang CC, Wu CH, Shieh JY, Wen CY, Ling EA. Immunohistochemical study of amoeboid microglial cells in fetal rat brain. J Anat. 1996; 189(3):567-574. (Biology). View Reference

すべて表示する (7)

554837 Rev. 12

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.