BV510 Rat Anti-Mouse CD4

BD Horizon™ BV510 Rat Anti-Mouse CD4

Name: BV510 Rat Anti-Mouse CD4
Brand: BV510 Rat Anti-Mouse CD4
SKU: 563106

クローン RM4-5 (also known as RM4.5) (RUO)

Two-color flow cytometric analysis of CD4 expression on mouse splenocytes. Splenic leucocytes from a BALB/c mouse were stained with PE Hamster Anti-Mouse CD3e antibody (Cat. No. 553064/553063/561824) and either BD Horizon™ BV510 Rat IgG2a, κ Isotype Control (Cat. No. 562952; Left Panel) or BD Horizon™ BV510 Rat Anti-Mouse CD4 antibody (Cat. No. 563106; Right Panel). Two-color flow cytometric dot plots show the correlated expression patterns of CD4 (or Ig Isotype control staining) versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.

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カタログ番号:563106

希望小売価格:¥54,450

単位:EA (1 Each)

容量:

50 µg

数量:

見積に追加

見積リストに保存バルク試薬見積の依頼

製品詳細

Brand: BD Horizon™

Alternative Name: Cd4; CD4 antigen; L3T4; Ly-4; T-cell surface antigen T4/Leu-3

Reactivity: Mouse (QC Testing)

Isotype: Rat DA, also known as DA/HA IgG2a, κ

Immunogen: Mouse Thymocytes (BALB/c)

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_2687550

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV510 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV510 were removed.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
An isotype control should be used at the same concentration as the antibody of interest.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Brilliant Violet™ 510 is a trademark of Sirigen.

データシート

Technical Data Sheet (TDS)

関連製品

Stain Buffer (FBS) RUO

サイズ 500 ML カタログ番号 554656

Lysing Buffer RUO

サイズ 100 ML カタログ番号 555899

BV510 Rat IgG2a, κ Isotype Control RUO

サイズ 50 µg カタログ番号 562952

PE Hamster Anti-Mouse CD3e RUO

サイズ 0.2 mg カタログ番号 553064

PE Hamster Anti-Mouse CD3e RUO

サイズ 0.1 mg カタログ番号 553063

PE Hamster Anti-Mouse CD3e RUO

サイズ 25 mg カタログ番号 561824

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563106 Rev. 1

抗体の詳細

RM4-5

The RM4-5 monoclonal antibody specifically binds to the CD4 (L3T4) differentiation antigen expressed on most thymocytes, subpopulations of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells and immunosuppressive regulatory T cells), and a subset of NK-T cells. CD4 has also been reported to be detected on pluripotent hematopoietic stem cells, bone marrow myeloid and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. CD4 has been reported to be expressed on the plasma membrane of mouse egg cells and is involved in adhesion of the egg to MHC class II-bearing sperm. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck. Purified RM4-5 mAb has been reported to block the binding of FITC-conjugated anti-mouse CD4 clones GK1.5 and H129.19, but not the RM4-4 clone.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.

563106 Rev. 1

フォーマットの詳細

BV510

The BD Horizon Brilliant Violet™ 510 (BV510) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye with an excitation maximum (Ex Max) at 327-nm / 405-nm and an emission maximum (Em Max) at 512-nm. BV510, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 510-nm (e.g., a 525/50 bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: BV510

最大励起波長: 327 nm, 405 nm

最大蛍光波長: 512 nm

563106 Rev.1

引用＆参考文献

View Development References

Development References (9)

Allman D, Li J, Hardy RR. Commitment to the B lymphoid lineage occurs before DH-JH recombination. J Exp Med. 1999; 189(4):735-740. (Biology). View Reference
Bendelac A. Mouse NK1+ T cells. Curr Opin Immunol. 1995; 7(3):367-374. (Biology). View Reference
Bierer BE, Sleckman BP, Ratnofsky SE, Burakoff SJ. The biologic roles of CD2, CD4, and CD8 in T-cell activation. Annu Rev Immunol. 1989; 7:579-599. (Biology). View Reference
Bosselut R, Zhang W, Ashe JM, Kopacz JL, Samelson LE, Singer A. Association of the adaptor molecule LAT with CD4 and CD8 coreceptors identifies a new coreceptor function in T cell receptor signal transduction. J Exp Med. 1999; 190(10):1517-1526. (Biology). View Reference
Godfrey DI, Kennedy J, Mombaerts P, Tonegawa S, Zlotnik A. Onset of TCR-β gene rearrangement and role of TCR-β expression during CD3-CD4-CD8- thymocyte differentiation. J Immunol. 1994; 152(10):4783-4792. (Clone-specific: Flow cytometry). View Reference
Nakamura T. Personal Communication. .
Wineman JP, Gilmore GL, Gritzmacher C, Torbett BE, Muller-Sieburg CE. CD4 is expressed on murine pluripotent hematopoietic stem cells. Blood. 1992; 180(7):1717-1724. (Biology). View Reference
Wu L, Antica M, Johnson GR, Scollay R, Shortman K. Developmental potential of the earliest precursor cells from the adult mouse thymus. J Exp Med. 1991; 174(6):1617-1627. (Biology). View Reference
Wu L, Scollay R, Egerton M, Pearse M, Spangrude GJ, Shortman K. CD4 expressed on earliest T-lineage precursor cells in the adult murine thymus. Nature. 1991; 349(6304):71-74. (Biology). View Reference

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563106 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.