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FITC Mouse Anti-Human CD57 HNK-1 体外診断用医薬品

FITC Mouse Anti-Human CD57 

Clone  HNK-1   (体外診断用医薬品)

  • Alternative Name Leu-7
  • Concentration 12.5 μg/mL
  • Vol. Per Test 20 μL
  • Isotype Mouse IgM, κ
  • Reactivity Human (QC Testing) 
  • Application Flow cytometry (Routinely Tested) 
  • Immunogen Membrane extracts of the HSB-2 T-lymphoblastoid cell line
  • Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
  • Regulatory Status 体外診断用医薬品
Product Details Recommended Assay References

Description

CD57, clone HNK-1, is derived from hybridization of mouse P3-X63-Ag8.653 myeloma cells with lymph node cells from BALB/c mice immunized with membrane extracts of the HSB-2 T–lymphoblastoid cell line.

CD57 (Leu-7) recognizes an Mr 110-kdalton (kDa) glycoprotein that is expressed on a subset of natural killer (NK) lymphocytes and T lymphocytes. The antigen is a carbohydrate structure associated with myelin-associated glycoprotein (MAG).

Format
  • Format FITC
  • Excitation Source Blue 488 nm
  • Excitation Max 494 nm
  • Emission Max 520 nm

FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.

Resources & Tools
 Spectrum Viewer  Panel Designer  Spectrum Viewer  Download TDS  Package Insert  Regulatory Document Website
Preparation and Storage

The monoclonal antibody is supplied as 25 µg purified immunoglobulin in 2.0 mL (12.5 µg/mL) of phosphate-buffered saline. The FITC conjugate is supplied as 25 µg in 2.0 mL (12.5 µg/mL). The biotin conjugate is supplied as 25 µg in 2.0 mL (12.5 µg/mL). Buffered saline contains gelatin and 0.1% sodium azide. The vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.


  1. Abo T, Balch CMA. A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). J Immunol. 1981;127:1024-1029.

  2. Bunn PA, Gazdar AF, Carney DN, Minna J. Small cell lung carcinoma and natural killer cells share an antigenic determinant, Leu-7. Clin Res. 1984;32:416A

  3. Kruse J, Mailhammer R, Wennecke H, et al. Neural cell-adhesion molecules and MAG share a common carbohydrate moiety recognized by monoclonal antibodies L2 and HNK-1. Nature. 1984;311:153-155.

  4. Lanier LL, Le AM, Civin C, Loken MR, Phillips JH. The relationship of CD16 (Leu-11) and Leu-19 (NKH-1) antigen expression on human peripheral blood NK cells and cytotoxic T lymphocytes. J Immunol. 1985;151:555.

  5. Lanier LL, Le AM, Phillips JH, Warner NL, Babcock GF. Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J Immunol. 1983;131:1789-1796.

  6. Lewis DE, Puck JM, Babcock GM, Rich RR. Disproportionate expansion of a minor T cell subset in patients with lymphadenopathy syndrome and acquired immunodeficiency syndrome. J Infect Disease. 1985;151:555.

  7. Manara GC, De Panfilis G, Ferrari C. Ultrastructural characterization of human large granular lymphocyte subsets defined by the expression of HNK-1 (Leu-7), Leu-11, or both HNK-1 and Leu-11 antigens. J Histochem Cytochem. 1985;33:1129-1133.

  8. McGarry RC, Helfand SL, Quarles RH, Roder JC. Recognition of myelin-associated glycoprotein by the monoclonal antibody HNK-1. Nature. 1983;306:376

  9. Mechtersheimer G, Staudter M, Moller P. Expression of the natural killer cell-associated antigens CD56 and CD57 in human neural and striated muscle cells and in their tumors. Cancer Res. 1991;51:1300-1307.

  10. Prince HE, Kreiss JK, Kasper CK, et al. Distinctive lymphocyte subpopulation abnormalities in patients with congenital coagulation disorders who exhibit lymph node enlargement. Blood. 1985;66:64.

  11. Schubert J, Lanier LL, Schmidt RE. Cluster report: CD57. In: Knapp W, Dörken B, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY; Oxford University Press; 1989:711-714.

  12. Stites D, Casavant C, McHugh T, et al. Flow cytometric analysis of lymphocyte phenotypes in AIDS using monoclonal antibodies and simultaneous dual immunofluorescence. Clin Immunol Immunopathol. 1986;38:161-177.

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