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FITC Mouse Anti-Human CD11a G-25.2 RUO (GMP)

FITC Mouse Anti-Human CD11a 

Clone  G-25.2   (RUO (GMP))

  • Alternative Name LFA-1α
  • Concentration 6.25 μg/mL
  • Vol. Per Test 20 μL
  • Isotype Mouse BALB/c IgG2a, κ
  • Reactivity Human (QC Testing) 
  • Application Flow cytometry (Routinely Tested) 
  • Immunogen Human cytotoxic T lymphocytes
  • Entrez Gene ID 3683 
  • Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
  • Regulatory Status RUO (GMP)
Product Details Recommended Assay References

Description

CD11a, clone G-25.2, is derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with human cytotoxic T lymphocytes (CTL).

CD11a (Anti-LFA-1α) recognizes the Mr 180-kdalton (kDa) α chain of the leucocyte function–associated antigen-1 (LFA-1). LFA-1, a member of a subfamily of integrin receptors, is a leucocyte adhesion receptor that binds to an intercellular adhesion molecule (ICAM) on target tissues. This subfamily consists of three heterodimeric proteins that share a common noncovalently-associated β chain (CD18 antigen, Mr 95 kDa) but possess distinct α-subunits recognized by CD11a (anti-LFA-1α), CD11b (Leu-15), and CD11c (Leu-M5).

Format
  • Format FITC
  • Excitation Source Blue 488 nm
  • Excitation Max 494 nm
  • Emission Max 520 nm

FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.

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Preparation and Storage

The monoclonal antibody is supplied as 25 µg purified immunoglobulin in 2.0 mL (12.5 µg/mL) of phosphate-buffered saline. The FITC conjugate is supplied as 12.5 µg in 2.0 mL (6.25 µg/mL). Buffered saline contains gelatin and 0.1% sodium azide. The vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.


  1. Anderson DC, Springer TA. Leukocyte adhesion deficiency: an inherited defect in Mac-1, LFA-1, and p150,95 glycoproteins. Annu Rev Med. 1987;38:175-194.

  2. Clayberger C, Wright A, Medeiros LJ, et al. Absence of cell surface LFA-1 as a mechanism of escape from immunosurveillance. Lancet. 1987;(Sept 5):533-536.

  3. Dustin ML, Springer TA. T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1. Nature. 1989;341:619-624.

  4. Fischer A, Griscelli C, Blanche S, et al. Prevention of graft failure by an anti-HLFA-1 monoclonal antibody in HLA-mismatched bonemarrow transplantation. Lancet. 1986;(Nov 8):1058-1061.

  5. Inghirami G, Wieczorek R, Zhu B-Y, Silber R, Dalla-Gavera R, Knowles DM. Differential expression of LFA-1 molecules in non-Hodgkin’s lymphoma and lymphoid leukemia. Blood. 1988;72:1431-1434.

  6. Mentzer SJ, Faller DV, Burakoff SJ. Interferon-γ induction of LFA-1–mediated homotypic adhesion of human monocytes. J Immunol. 1986;137:108-113

  7. Uciechowski P, Schmidt RE. Cluster report: CD11. In: Knapp W, Dörken W, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford: University Press; 1989:543.

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