BUV395 Rat Anti-Mouse TER-119/Erythroid Cells

BD Horizon™ BUV395 Rat Anti-Mouse TER-119/Erythroid Cells

Name: BUV395 Rat Anti-Mouse TER-119/Erythroid Cells
Brand: BUV395 Rat Anti-Mouse TER-119/Erythroid Cells
SKU: 566206

クローン TER-119 (RUO)

Two-color flow cytometric analysis of TER-119/Erythroid Cells expressed on mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) antibody (Cat. No. 553141/553142). The cells were then stained with PE Rat Anti-Mouse CD45 antibody (Cat. No. 553081/561087) and either BD Horizon™ BUV395 Rat IgG2b, κ Isotype Control (Cat. No. 563560; Left Panel) or BD Horizon™ BUV395 Rat Anti-Mouse TER-119/Erythroid Cells antibody (Cat. No. 563827; Right Panel). Two-color flow cytometric dot plots showing the expressed levels of CD45 versus TER-119 (or Ig isotype control staining) were derived from gated events with the forward and side-scattered light characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometry System.

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カタログ番号:566206

希望小売価格:¥30,250

単位:EA (1 Each)

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製品詳細

Brand: BD Horizon™

Alternative Name: Lymphocyte antigen 76; Ly76; Ly-76; TER-119; Ter119

Reactivity: Mouse (QC Testing)

Isotype: Rat WI, also known as Wistar (outbred) IgG2b, κ

Immunogen: Mouse Fetal Liver

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_2738438

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV395 under optimum conditions, and unconjugated antibody and free BD Horizon BUV395 were removed.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

データシート

Technical Data Sheet (TDS)

サイズ 0.1 mg カタログ番号 553141

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO

サイズ 0.5 mg カタログ番号 553142

Stain Buffer (FBS) RUO

サイズ 500 ML カタログ番号 554656

BUV395 Rat IgG2b, κ Isotype Control RUO

サイズ 50 µg カタログ番号 563560

PE Rat Anti-Mouse CD45 RUO

サイズ 0.1 mg カタログ番号 553081

PE Rat Anti-Mouse CD45 RUO

サイズ 25 mg カタログ番号 561087

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566206 Rev. 1

抗体の詳細

TER-119

The TER-119 antibody specifically binds to a 52 kDa molecule associated with glycophorin A on cells of the erythroid lineage in embryonic yolk sac, fetal liver, newborn liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs. The TER-119 antigen is expressed on erythroid cells from pro-erythroblast through mature erythrocyte stages, but not on cells with BFU-E or CFU-E activities. The TER-119 epitope is not detected on hematopoietic stem cells, lymphoid cells, myeloid cells, or erythroleukemia lines. The TER-119 mAb is a component of the "lineage cocktail" used in studies of hematopoietic progenitors to detect, or deplete cells committed to the hematopoietic lineages.

The antibody was conjugated to BD Horizon™ BUV395 which has been exclusively developed by BD Biosciences as an optimal dye for use on a 355 nm laser equipped instrument. With an Ex Max at 348 nm and an Em Max at 395 nm, this dye has virtually no spillover into any other detector. BD Horizon™ BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter.

566206 Rev. 1

フォーマットの詳細

BUV395

The BD Horizon Brilliant™ Ultraviolet 395 (BUV395) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This base dye is a polymer fluorochrome with an excitation maximum (Ex Max) of 348-nm and an emission maximum (Em Max) at 395-nm. Driven by BD innovation, BUV395 is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 380-nm (e.g., 379/28-nm bandpass filter). BUV395 is the ideal dye when using only one detector on the ultraviolet laser as it spills into no other detectors and no other fluors spill into it. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: BUV395

最大励起波長: 348 nm

最大蛍光波長: 395 nm

566206 Rev.1

引用＆参考文献

View Development References

Development References (5)

Ikuta K, Kina T, MacNeil I, et al. A developmental switch in thymic lymphocyte maturation potential occurs at the level of hematopoietic stem cells. Cell. 1990; 62(5):863-874. (Clone-specific). View Reference
Kina T, Ikuta K, Takayama E, et al. The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage. Br J Haematol. 2000; 109(2):280-287. (Immunogen: Immunoprecipitation, Western blot). View Reference
Kitajima K, Kojima M, Nakajima K, et al. Definitive but not primitive hematopoiesis is impaired in jumonji mutant mice. Blood. 1999; 93(1):87-95. (Clone-specific: Flow cytometry, Immunohistochemistry). View Reference
Maraskovsky E, Brasel K, Teepe M, et al. Dramatic increase in the numbers of functionally mature dendritic cells in Flt3 ligand-treated mice: multiple dendritic cell subpopulations identified. J Exp Med. 1996; 184(5):1953-1962. (Clone-specific: Cell separation, Cytotoxicity, Depletion, Flow cytometry). View Reference
Osawa M, Tokumoto Y, Nakauchi H. Hematopoietic stem cells. In: Herzenberg LA, Weir DM, Blackwell C, ed. Weir's Handbook of Experimental Immunology, 5th Edition. Cambridge: Blackwell Science; 1996:66.1-66.5.

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566206 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

BD Horizon™ BUV395 Rat Anti-Mouse TER-119/Erythroid Cells

クローン TER-119 (RUO)

Preparation and Storage

Product Notices

関連製品

Development References (5)