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FITC Mouse Anti-Human CD15 MMA 体外診断用医薬品

FITC Mouse Anti-Human CD15 

Clone  MMA   (体外診断用医薬品)

  • Concentration 100 μg/mL
  • Vol. Per Test 20 μL
  • Isotype Mouse BALB/c IgM, κ
  • Reactivity Human (QC Testing) 
  • Application Flow cytometry (Routinely Tested) 
  • Immunogen U-937 histiocytic cell line
  • Entrez Gene ID 2526 
  • Storage Buffer HEPES buffer with gelatin and 0.1% sodium azide.
  • Regulatory Status 体外診断用医薬品
Product Details Recommended Assay References

Description

CD15, clone MMA, is derived from the hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with the U-937 histiocytic cell line.

CD15 recognizes a human myelomonocytic antigen. The structure recognized by CD15 antibodies is lacto-N-fucopentose III.

Format
  • Format FITC
  • Excitation Source Blue 488 nm
  • Excitation Max 494 nm
  • Emission Max 520 nm

FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.

Resources & Tools
 Spectrum Viewer  Panel Designer  Spectrum Viewer  Package Insert  Download TDS  Regulatory Document Website
Preparation and Storage

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.


  1. Hanjan SN, Kearney JF, Cooper MD. A monoclonal antibody (MMA) that identi es a differentiation antigen on human myelomonocytic cells. Clin Immunol Immunopathol. 1982;23:172-188.

  2. Hsu SM, Jaffe ES. Leu-M1 and peanut agglutinin stain the neoplastic cells of Hodgkin's Disease. Amer J Clin Path. 1984;82:29.

  3. Pinkus GS, Thomas P, Said JW. Leu-M1—A marker for Reed-Sternberg cells in Hodgkin's disease: an immunoperoxidase study of paraffin-embedded tissues. Am J Pathol. 1985;119:244.

  4. Skubitz K, Balke J, Ball E, et al. Report on the CD15 cluster workshop. In: Knapp W, Dörken B, Gilks W, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press Inc; 1989:800-805.

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