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      PE-CF594 Rat Anti-Mouse CD135
      PE-CF594 Rat Anti-Mouse CD135
      Multicolor flow cytometric analysis of CD135 expression on C57BL/6 bone marrow cells. Mouse bone marrows cells from C57BL/6 mice were stained with BD Pharmingen™ Brilliant Violet™ 421 Rat Anti-Mouse CD117 antibody (Cat. No. 562609) and BD Horizon™ PE-CF594 Rat IgG2a, κ Isotype Control (Cat. No. 562302, Left Panel) or BD Horizon™ PE-CF594 Rat Anti-Mouse CD135 antibody (Cat. No. 562537, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of CD135 (or Ig Isotype Control background staining) versus CD117 were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
      PE-CF594 Rat Anti-Mouse CD135
      Multicolor flow cytometric analysis of CD135 expression on C57BL/6 bone marrow cells. Mouse bone marrows cells from C57BL/6 mice were stained with BD Pharmingen™ Brilliant Violet™ 421 Rat Anti-Mouse CD117 antibody (Cat. No. 562609) and BD Horizon™ PE-CF594 Rat IgG2a, κ Isotype Control (Cat. No. 562302, Left Panel) or BD Horizon™ PE-CF594 Rat Anti-Mouse CD135 antibody (Cat. No. 562537, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of CD135 (or Ig Isotype Control background staining) versus CD117 were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
      製品詳細
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      BD Horizon™
      Flt3; Fms-like tyrosine kinase 3; FLT-3; FLK-2; Fetal liver kinase 2; Ly72
      Mouse (QC Testing)
      Rat WI, also known as Wistar (outbred) IgG2a, κ
      Mouse Flt-3 Transfected Cell Line
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      14255
      AB_2737639
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation and Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ PE-CF594 under optimum conditions, and unconjugated antibody and free PE-CF594 were removed.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. An isotype control should be used at the same concentration as the antibody of interest.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      5. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
      6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      8. Texas Red is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      9. CF™ is a trademark of Biotium, Inc.
      10. When excited by the yellow-green (561-nm) laser, the fluorescence may be brighter than when excited by the blue (488-nm) laser.
      11. This product is provided under an Agreement between BIOTIUM and BD Biosciences. The manufacture, use, sale, offer for sale, or import of this product is subject to one or more patents or pending applications owned or licensed by Biotium, Inc. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
      12. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using multi-laser cytometers, which may directly excite both PE and CF™594.
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      562537 Rev. 1
      抗体の詳細
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      A2F10.1

      The A2F10 monoclonal antibody specifically binds to Flk-2/Flt3 (Ly-72, CD135), a receptor protein tyrosine kinase closely related to c-kit, c-fms, and PDGF Receptor of the immunoglobulin superfamily. The Flt3 message is detected in hematopoietic stem cells and primitive progenitor cells in fetal liver, adult bone marrow, and fetal and adult thymus, as well as brain, placenta, and testis; but it is absent in more mature hematopoietic cells. In flow cytometric analysis, the A2F10 antibody recognizes Flt3-transfected Y3 cells (rat myeloma), but not the parent cell line in addition to recognizing early B lymphoid lineage cells in juvenile and adult bone marrow. A role for CD135 in the regulation of hematopoiesis is suggested by the observations that soluble Flk-2/Flt3 ligand can both stimulate proliferation of stem cell-enriched fetal liver, fetal thymus, and adult bone marrow populations and enhance their responses to other growth factors in vitro. In addition, injection of Flk-2/Flt3 ligand stimulates extramedullary hematopoiesis in the mouse spleen and accumulation of dendritic cells in the hematopoietic system.  mAb A2F10.1 is reported to immunoprecipitate a 150-kDa surface protein from the murine myeloblast cell line M1, which naturally expresses CD135, and to inhibit the binding of Flk-2/Flt3 ligand to CD135.

      This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg 610/20-nm filter).

      562537 Rev. 1
      フォーマットの詳細
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      PE-CF594
      BD Horizon™ PE-CF594 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye with an emission maximum (Em Max) at 615-nm. PE-CF594, driven by BD innovation, is designed to be excited by the blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 615 nm (e.g., a 610/20-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the green (532-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE-CF594
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      615 nm
      562537 Rev.1
      引用＆参考文献
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      Development References (10)

      1. Hannum C, Culpepper J, Campbell D, et al. Ligand for FLT3/FLK2 receptor tyrosine kinase regulates growth of haematopoietic stem cells and is encoded by variant RNAs. Nature. 1994; 368(2):643-648. (Biology). View Reference
      2. Lyman SD, James L, Vanden Bos T, et al. Molecular cloning of a ligand for the flt3/flk-2 tyrosine kinase receptor: a proliferative factor for primitive hematopoietic cells. Cell. 1993; 75(6):1157-1167. (Biology). View Reference
      3. Matthews W, Jordan CT, Wiegand GW, Pardoll D, Lemischka IR. A receptor tyrosine kinase specific to hematopoietic stem and progenitor cell-enriched populations. Cell. 1991; 65(7):1143-1152. (Biology). View Reference
      4. Ogawa M, Sugawara S, Kunisada T, et al. Flt3/Flk-2 and c-Kit are not essential for the proliferation of B lymphoid progenitor cells in the bone marrow of the adult mouse. Exp Hematol. 1998; 26(6):478-488. (Clone-specific). View Reference
      5. Ogawa M, ten Boekel E, Melchers F. Identification of CD19(-)B220(+)c-Kit(+)Flt3/Flk-2(+)cells as early B lymphoid precursors before pre-B-I cells in juvenile mouse bone marrow. Int Immunol. 2000; 12(3):313-324. (Biology). View Reference
      6. Orlic D, Fischer R, Nishikawa S, Nienhuis AW, Bodine D. Purification and characterization of heterogeneous pluripotent hematopoietic stem cell populations expressing high levels of c-kit receptor. Blood. 1993; 82(3):762-770. (Biology). View Reference
      7. Shurin MR, Pandharipande PP, Zorina TD, et al. FLT3 ligand induces the generation of functionally active dendritic cells in mice. Cell Immunol. 1997; 179(2):174-184. (Biology). View Reference
      8. Veiby OP, Jacobsen FW, Cui L, Lyman SD, Jacobsen SE. The flt3 ligand promotes the survival of primitive hemopoietic progenitor cells with myeloid as well as B lymphoid potential. Suppression of apoptosis and counteraction by TNF-alpha and TGF-beta. J Immunol. 1996; 157(7):2953-2960. (Biology). View Reference
      9. Veiby OP, Lyman SD, Jacobsen SE. Combined signaling through interleukin-7 receptors and flt3 but not c-kit potently and selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells.. Blood. 1996; 88(4):1256-65. (Biology). View Reference
      10. Wasserman R, Li YS, Hardy RR. Differential expression of the blk and ret tyrosine kinases during B lineage development is dependent on Ig rearrangement. J Immunol. 1995; 155(2):644-651. (Biology). View Reference
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      562537 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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