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FITC Rat Anti-Mouse CD16/CD32 2.4G2 RUO

FITC Rat Anti-Mouse CD16/CD32 

Clone  2.4G2   (RUO)

  • Brand BD Pharmingen™
  • Alternative Name FcγRIII/FcγRII; Fcgr3/Fcgr2
  • Concentration 0.5 mg/ml
  • Isotype Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
  • Reactivity Mouse (QC Testing) 
  • Application Flow cytometry (Routinely Tested) 
  • Immunogen Mouse BALB/c Macrophage J774
  • Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The 2.4G2 antibody reacts specifically with a common nonpolymorphic epitope on the extracellular domains of the mouse FcγIII and FcγII receptors. It has also been reported to bind the FcγI receptor (CD64) via its Fc domain. 2.4G2 mAb blocks non-antigen-specific binding of immunoglobulins to the FcγIII and FcγII, and possibly FcγI, receptors in vitro and in vivo. CD16 and/or CD32 are expressed on natural killer cells, monocytes, macrophages, dendritic cells (at low levels), Kupffer cells, granulocytes, mast cells, B lymphocytes, immature thymocytes, and some activated mature T lymphocytes.

Format
  • Format FITC
  • Excitation Source Blue 488 nm
  • Excitation Max 494 nm
  • Emission Max 520 nm

FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.

Other Formats →

Suggested Companion Products

PE Hamster Anti-Mouse CD3e 145-2C11 RUO
0.1 mg Cat No: 553063

Stain Buffer (FBS) RUO
500 mL Cat No: 554656

FITC Rat IgG2b, κ Isotype Control RUO
0.25 mg Cat No: 553988

Resources & Tools
 Spectrum Viewer  Panel Designer  Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. An isotype control should be used at the same concentration as the antibody of interest.


  1. Araujo-Jorge T, Rivera MT, el Bouhdidi A, Daeron M, Carlier Y. An Fc gamma RII-, Fc gamma RIII-specific monoclonal antibody (2.4G2) decreases acute Trypanosoma cruzi infection in mice. Infect Immun. 1993; 61(11):4925-4928. (Clone-specific: Flow cytometry). View reference

  2. Benhamou M, Bonnerot C, Fridman WH, Daeron M. Molecular heterogeneity of murine mast cell Fc gamma receptors. J Immunol. 1990; 144(8):3071-3077. (Clone-specific: Flow cytometry). View reference

  3. Jensen WA, Marschner S, Ott VL, Cambier JC. FcgammaRIIB-mediated inhibition of T-cell receptor signal transduction involves the phosphorylation of SH2-containing inositol 5-phosphatase (SHIP), dephosphorylation of the linker of activated T-cells (LAT) and inhibition of calcium mobilization. Biochem Soc Trans. 2001; 29(6):840-846. (Biology: Flow cytometry). View reference

  4. Katz HR, Arm JP, Benson AC, Austen KF. Maturation-related changes in the expression of Fc gamma RII and Fc gamma RIII on mouse mast cells derived in vitro and in vivo. J Immunol. 1990; 145(10):3412-3417. (Clone-specific: Flow cytometry). View reference

  5. Kurlander RJ, Ellison DM, Hall J. The blockade of Fc receptor-mediated clearance of immune complexes in vivo by a monoclonal antibody (2.4G2) directed against Fc receptors on murine leukocytes. J Immunol. 1984; 133(2):855-862. (Clone-specific: Flow cytometry). View reference

  6. Lewis VA, Koch T, Plutner H, Mellman I. A complementary DNA clone for a macrophage-lymphocyte Fc receptor. Nature. 1986; 324(6095):372-375. (Clone-specific: Flow cytometry). View reference

  7. Maeda K, Burton GF, Padgett DA, et al. Murine follicular dendritic cells and low affinity Fc receptors for IgE (Fc epsilon RII). J Immunol. 1992; 148(8):2340-2347. (Clone-specific: Flow cytometry). View reference

  8. Mellman IS, Unkeless JC. Purificaton of a functional mouse Fc receptor through the use of a monoclonal antibody. J Exp Med. 1980; 152(4):1048-1069. (Clone-specific: Flow cytometry). View reference

  9. Perussia B, Tutt MM, Qiu WQ, et al. Murine natural killer cells express functional Fc gamma receptor II encoded by the Fc gamma R alpha gene. J Exp Med. 1989; 170(1):73-86. (Clone-specific: Flow cytometry). View reference

  10. Ravetch JV, Luster AD, Weinshank R, et al. Structural heterogeneity and functional domains of murine immunoglobulin G Fc receptors. Science. 1986; 234(4777):718-725. (Clone-specific: Flow cytometry). View reference

  11. Rodewald HR, Awad K, Moingeon P, et al. Fc gamma RII/III and CD2 expression mark distinct subpopulations of immature CD4-CD8- murine thymocytes: in vivo developmental kinetics and T cell receptor beta chain rearrangement status. J Exp Med. 1993; 177(4):1079-1092. (Biology: Flow cytometry). View reference

  12. Rodewald HR, Moingeon P, Lucich JL, Dosiou C, Lopez P, Reinherz EL. A population of early fetal thymocytes expressing Fc gamma RII/III contains precursors of T lymphocytes and natural killer cells. Cell. 1992; 69(1):139-150. (Clone-specific: Flow cytometry). View reference

  13. Takezawa R, Watanabe Y, Akaike T. Direct evidence of macrophage differentiation from bone marrow cells in the liver: a possible origin of Kupffer cells. J Biochem (Tokyo). 1995; 118(6):1175-1183. (Biology: Flow cytometry). View reference

  14. Titus JA, Finkelman FD, Stephany DA, Jones JF, Segal DM. Quantitative analysis of Fc gamma receptors on murine spleen cell populations by using dual parameter flow cytometry. J Immunol. 1984; 133(2):556-561. (Biology: Flow cytometry). View reference

  15. Unkeless JC. Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors. J Exp Med. 1979; 150(3):580-596. (Immunogen: Flow cytometry). View reference

  16. Vremec D, Zorbas M, Scollay R, et al. The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells. J Exp Med. 1992; 176(1):47-58. (Biology: Flow cytometry). View reference

  17. Witmer MD, Steinman RM. The anatomy of peripheral lymphoid organs with emphasis on accessory cells: light-microscopic immunocytochemical studies of mouse spleen, lymph node, and Peyer's patch. Am J Anat. 1984; 170(3):465-481. (Clone-specific: Flow cytometry). View reference

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