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FITC Mouse Anti-BrdU B44 RUO

FITC Mouse Anti-BrdU 

Clone  B44   (RUO)

  • Concentration 25 μg/mL
  • Vol. Per Test 20 μL
  • Isotype Mouse BALB/c IgG1, κ
  • Application Flow cytometry (Routinely Tested) 
  • Immunogen Iodouridine
  • Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

Anti-BrdU, clone B44, is derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with spleen cells from BALB/c mice immunized with iodouridine-conjugated ovalbumin.

Bromodeoxyuridine (BrdU) is a uridine derivative that can be incorporated into DNA in place of thymidine. Anti-BrdU identifies BrdU (but not thymidine) in single-stranded DNA, free BrdU, or BrdU coupled to a protein carrier. The antibody also reacts with iodouridine.

Format
  • Format FITC
  • Excitation Source Blue 488 nm
  • Excitation Max 494 nm
  • Emission Max 520 nm

FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.

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Preparation and Storage

The monoclonal antibody is supplied as 50 µg purified immunoglobulin in 2.0 mL (25 µg/mL) of phosphate-buffered saline. The FITC conjugate is supplied as 50 µg in 2.0 mL (25 µg/mL). Buffered saline contains gelatin and 0.1% sodium azide. The vials should be stored at 2º to 8ºC. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.


  1. Beisker W, Dolbeare F, Gray JW. An improved immunocytochemical procedure for high-sensitivity detection of incorporated bromodeoxyuridine. Cytometry. 1987;8:235.

  2. Dolbeare F, Beisker W, Pallavicini MG, Vanderlaan M, Gray JW. Cytochemistry for bromodeoxyuridine/DNA analysis: Stoichiometry and sensitivity. Cytometry. 1985;6:521-530.

  3. Dolbeare F, Gratzner HG, Pallavicini MG, Gray JW. Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. Proc Natl Acad Sci USA. 1985;6:521-530.

  4. Gratzner HG. Monoclonal antibody to 5-bromo and 5-iododeoxyuridine: A new reagent for detection of DNA replication. Science. 1982;218:474.

  5. Gray JW. Monoclonal antibodies against bromodeoxyuridine (special issue). Science. 1985;6:501-673.

  6. Nagashima T, Hoshino T. Rapid detection of S-phase cells by anti-bromodeoxyuridine monoclonal antibody in 9L brain tumor cells in vitro and in situ. Acta Neuropathol (Berl). 1985;66:12.

  7. Pinkel D, Thompson LH, Gray JW, Vanderlaan M. Measurement of sister chromatid exchanges at very low bromodeoxyuridine substitution levels using a monoclonal antibody in Chinese hamster ovary cells. Cancer Res. 1985;45:5795.

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