FITC Mouse Anti-BrdU

BD™ FITC Mouse Anti-BrdU

Name: FITC Mouse Anti-BrdU
Brand: FITC Mouse Anti-BrdU
SKU: 347583

クローン B44 (RUO)

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カタログ番号:347583

希望小売価格:¥94,000

単位:EA (1 Each)

容量:

100 Tests

数量:

見積に追加

見積リストに保存バルク試薬見積の依頼

製品詳細

Brand: BD™

Isotype: Mouse IgG1

Immunogen: Iodouridine

Application: Flow cytometry, Intracellular staining (flow cytometry)

Concentration: 25 μg/mL

Vol. Per Test: 20 μL

RRID: AB_400327

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO

Preparation and Storage

The monoclonal antibody is supplied as 50 µg purified immunoglobulin in 2.0 mL (25 µg/mL) of phosphate-buffered saline. The FITC conjugate is supplied as 50 µg in 2.0 mL (25 µg/mL). Buffered saline contains gelatin and 0.1% sodium azide. The vials should be stored at 2º to 8ºC. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

データシート

Technical Data Sheet (TDS)

347583 Rev. 1

抗体の詳細

B44

The Anti-BrdU antibody, clone B44, is derived from hybridization of Sp2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice immunized with iodouridine-conjugated ovalbumin.

Bromodeoxyuridine (BrdU) is a uridine derivative that can be incorporated specifically into DNA in place of thymidine. Anti-BrdU identifies BrdU (but not thymidine) in single-stranded DNA, free BrdU, or BrdU coupled to a protein carrier. The antibody also reacts with iodouridine. DNase-I cleaves each strand of DNA at random and permits the binding of Anti-BrdU antibody to its antigen (BrdU).

347583 Rev. 1

フォーマットの詳細

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: FITC

最大励起波長: 494 nm

最大蛍光波長: 518 nm

347583 Rev.1

引用＆参考文献

View Development References

Development References (7)

Beisker W, Dolbeare F, Gray JW. An improved immunocytochemical procedure for high-sensitivity detection of incorporated bromodeoxyuridine. Cytometry. 1987; 8:235. (Biology).
Dolbeare F, Beisker W, Pallavicini MG, Vanderlaan M, Gray JW. Cytochemistry for bromodeoxyuridine/DNA analysis: Stoichiometry and sensitivity. Cytometry. 1985; 6:521-530. (Biology).
Dolbeare F, Gratzner H, Pallavicini MG, Gray JW. Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. Proc Natl Acad Sci U S A. 1983; 80(18):5573-5577. (Biology). View Reference
Gratzner HG. Monoclonal antibody to 5-bromo and 5-iododeoxyuridine: A new reagent for detection of DNA replication. Science. 1982; 218:474. (Biology).
Gray JW. Monoclonal antibodies against bromodeoxyuridine (special issue). Science. 1985; 6:501-673. (Biology).
Nagashima T, Hoshino T. Rapid detection of S-phase cells by anti-bromodeoxyuridine monoclonal antibody in 9L brain tumor cells in vitro and in situ. Acta Neuropathol (Berl). 1985; 66:12. (Biology).
Pinkel D, Thompson LH, Gray JW, Vanderlaan M. Measurement of sister chromatid exchanges at very low bromodeoxyuridine substitution levels using a monoclonal antibody in Chinese hamster ovary cells. Cancer Res. 1985; 45:5795. (Biology).

すべて表示する (7)

347583 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.