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CD10 BV421 HI10a RUO

CD10 BV421 

Clone  HI10a   (RUO)

  • Brand BD Horizon™
  • Concentration 6.3 μg/mL
  • Vol. Per Test 5 μL
  • Isotype Mouse BALB/c IgG1, κ
  • Reactivity Human (QC Testing) 
  • Application Flow cytometry (Routinely Tested) 
  • Immunogen Acute CALLA Leukemia Blast Cells
  • Workshop No. V CD10.7
  • Entrez Gene ID 4311 
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The CD10 antibody, clone HI10a, is derived from the hybridization of mouse P3-63-Ag8.653 myeloma cells with spleen cells of BALB/c mice immunized with blasts from a patient with acute CALLA leukemia.

The CD10 antibody recognizes a 100-kilodalton (kDa) type II transmembrane, glycosylated, zinc-containing metalloprotease. The CD10 antigen is also known as common acute lymphoblastic leukemia antigen (CALLA), neutral endopeptidase (NEP), gp100, and enkephalinase.

Format
  • Format BV421
  • Excitation Source Violet 405 nm
  • Excitation Max 407 nm
  • Emission Max 421 nm

BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.

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  1. Protection of Laboratory Workers from Occupationally Acquired Infections; Approved
    Guideline—Third Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2005. CLSI
    document M29-A3.

  2. Caligaris-Cappio F, Riva M, Tesio L, Schena M, Gaidano G, Bergui L. Human normal CD5+ B
    lymphocytes can be induced to differentiate to CD5– B lymphocytes with germinal center cell features.
    Blood. 1989;73:1259-1263.

  3. Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal
    precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other
    bloodborne pathogens in health-care settings. MMWR. 1988;37:377-388.

  4. Connelly JC, Chambless R, Holiday D, Chittenden K, Johnson AR. Up-regulation of neutral
    endopeptidase (CALLA) in human neutrophils by granulocyte-macrophage colony-stimulating factor. J
    Leukoc Biol. 1993;53:685-690.

  5. Connelly JC, Skidgel RA, Schulz WW, Johnson AR, Erdos EG. Neutral endopeptidase 24.11 in human
    neutrophils: cleavage of chemotactic peptide. Proc Natl Acad Sci USA. 1985;82:8737-8741.

  6. Consolini R, Legitimo A, Rondelli R, et al. Clinical relevance of CD10 expression in childhood ALL.
    Haematologica. 1998;83:967-973.

  7. Erdös EG, Skidgel RA. Neutral endopeptidase 24.11 (enkephalinase) and related regulators of peptide
    hormones. FASEB J. 1989;3:145-151.

  8. Erdös EG, Wagner B, Harbury CB, Painter RG, Skidgel RA, Fa XG. Down-regulation and inactivation of
    neutral endopeptidase 24.11 (enkephalinase) in human neutrophils. J Biol Chem.
    1989;264:14519-14523.

  9. Greaves MF. Monoclonal antibodies as probes for leukemic heterogeneity and hematopoietic
    differentiation. In: Knapp W, ed. Leukemia Markers. New York, NY: Academic Press; 1981:19.

  10. Hofman P, Selva E, Le Negrate G, et al. CD10 inhibitors increase f-Met-Leu-Phe-induced neutrophil
    transmigration. J Leukoc Biol. 1998;63:312-320.

  11. LeBien TW, McCormack RT. The common acute lymphoblastic leukemia antigen (CD10)—emancipation
    from a functional enigma. Blood. 1989;73:625-635.

  12. Letarte M, Vera S, Tran R, et al. Common acute lymphocytic leukemia antigen is identical to neutral
    endopeptidase. J Exp Med. 1988;168:1247-1253.

  13. Salles G, Rodewald HR, Chin BS, Reinherz EL, Shipp MA. Inhibition of CD10/neutral endopeptidase
    24.11 promotes B-cell reconstitution and maturation in vivo. Proc Natl Acad Sci USA.
    1993;90:7618-7622.

  14. Zola H. CD10 Workshop Panel report. In: Schlossman SF, Boumsell L, Gilks W, et al, eds. Leucocyte
    Typing V: White Cell Differentiation Antigens. Vol 1. New York, NY: Oxford University Press;
    1995:505-507.

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