Size:
1 mg
Status:
RUO
Cat No:
565082
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The BD Horizon™ Cell Proliferation Dyes are non-fluorescent molecules that passively diffuse into cells. Once inside the cell, they are hydrolyzed by intracellular non-specific esterases to become fluorescent products. After hydrolysis, the dye becomes trapped inside the cell.
BD Horizon™ Human T Cell Backbone Panel
The BD Horizon™ Cell Proliferation Dyes are non-fluorescent molecules that passively diffuse into cells. Once inside the cell, they are hydrolyzed by intracellular nonspecific esterases to become fluorescent products. After hydrolysis, the dye becomes trapped inside the cell.
We address considerations and demonstrate the effects of the VPD450 dye on the ability of cells to proliferate or respond to activation signals by techniques such as BrdU incorporation, cytokine production, and cell signaling.
Screen a variety of cancer cell lines for relative expression of target antigens. Assess levels of phagocytosis mediated by macrophages.
BD Accuri™ C6 Plus Flow Cytometer Optical Filter Guide
Sorting and downstream functional assessment of regulatory T cells isolating live cells with the BD FACSMelody™ Cell Sorter
CFSE - TDS 565082
BD Accuri™ C6 Plus Fluorochrome Reference
Immunological applications
Isolating live cells
The balance of cell proliferation and apoptosis is important for both development and normal tissue homeostasis. Cell proliferation is an increase in the number of cells as a result of growth and division. Cell proliferation is regulated by the cell cycle, which is divided into a series of phases. Apoptosis, or programmed cell death, results in controlled self-destruction.
BD Horizon™ Human T Cell Backbone Panel - eBook 568263
BD Accuri™ C6 Plus Flow Cytometer Brochure
Violet Proliferation Dye 450 - TDS 562158