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Purified Mouse Anti-TNIK
製品詳細
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Human TNIK aa. 522-644
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
150 kDa
250 µg/ml
AB_399573
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612250 Rev. 1
抗体の詳細
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53/TNIK

Ste20 is a S. cerevisiae Ser/Thr protein kinase that functions upstream of the MAP kinase module. Mammalian and yeast homologs of this kinase are divided into two classes based on their structure and regulation. Members of the first class (Ste20, Cla4, and p21-activated protein kinase) contain a C-terminal kinase domain, an N-terminal regulatory domain and a small GTPase Rac1/Cdc42 binding domain. Members of the second class lack the GTPase binding sites, but are similar to the former class throughout the catalytic domain. This second class includes germinal center kinase (GCK), HPK, KHS, KRS1 and 2, MST1, 2, and 3, NIK, SOK-1, and TNIK. Traf2- and NCK-interacting kinase (TNIK) is most homologous to another NCK-interacting kinase, NIK. TNIK contains an N-  terminal kinase domain, and a C-terminal GCK homology (GCKH) domain. The mRNA of TNIK is expressed highest in heart, brain, and skeletal muscle. Overexpression of TNIK activates the JNK pathway, and leads to the disruption of F-actin structures and the inhibition of cell spreading. In vitro, TNIK can phosphorylate gelsolin. Thus, TNIK is a GCK family kinase that may regulate both the JNK pathway and cytoskeletal dynamics.

612250 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612250 Rev.1
引用&参考文献
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Development References (1)

  1. Fu CA, Shen M, Huang BC, Lasaga J, Payan DG, Luo Y. TNIK, a novel member of the germinal center kinase family that activates the c-Jun N-terminal kinase pathway and regulates the cytoskeleton. J Biol Chem. 1999; 274(43):30729-30737. (Biology). View Reference
612250 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.