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      Purified Mouse Anti-Human IKKβ w/ Control

      BD Pharmingen™ Purified Mouse Anti-Human IKKβ w/ Control

      (RUO)
      Purified Mouse Anti-Human IKKβ w/ Control
      Western blot analysis of Ikkβ. Lysate from HeLa cells was probed with anti-Ikkβ (clone F18-1875, Cat. No. 51-8120GR) at concentrations of 0.25 (lane 1), 0.125 (lane 2), and 0.06 µg/ml (lane 3). Ikkβ is identified as a band of ~87 kDa.
      Purified Mouse Anti-Human IKKβ w/ Control Purified Mouse Anti-Human IKKβ w/ Control
      Western blot analysis of Ikkβ. Lysate from HeLa cells was probed with anti-Ikkβ (clone F18-1875, Cat. No. 51-8120GR) at concentrations of 0.25 (lane 1), 0.125 (lane 2), and 0.06 µg/ml (lane 3). Ikkβ is identified as a band of ~87 kDa.
      製品詳細
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      BD Pharmingen™
      Human (QC Testing)
      Western blot (Routinely Tested), Immunoprecipitation (Tested During Development)
      RUO
      AB_647275


      推奨アッセイ手順

      Applications include western blot analysis (0.06 - 0.25 µg/ml). Other applications not routinely tested at BD Biosciences Pharmingen include immunoprecipitation (4 µg/sample). Store the antibody at -20°C. HeLa control lysate [50 µg (1 µg/µl)] is provided as a positive control (Cat. No. 51-16516N; store lysate at -20°C). HeLa control lysate (Cat. No. 611449) is sold separately as a ready-to-use western blot control.

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      製品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
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      551920 Rev. 7
      構成品
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      説明 数量/容量 Part Number EntrezGene ID
      Purified Mouse Anti-Human IKKβ 50 µg (1 ea) 51-8120GR N/A
      HeLa Control Lysate 50 µg (1 ea) 51-16516N N/A
      551920 Rev. 7
      引用＆参考文献
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      開発者向け参考資料 (11)

      1. DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB. Nature. 1997; 388(6642):548-554. (Biology). 参考文献を見る
      2. Israël A. The IKK complex: an integrator of all signals that activate NF-kappaB. Trends Cell Biol. 2000; 10(4):129-133. (Biology). 参考文献を見る
      3. Lee FS, Hagler J, Chen ZJ, Maniatis T. Activation of the IkappaB alpha kinase complex by MEKK1, a kinase of the JNK pathway. Cell. 1997; 88(2):213-222. (Biology). 参考文献を見る
      4. Li Q, Van Antwerp D, Mercurio F, Lee KF, Verma IM. Severe liver degeneration in mice lacking the IkappaB kinase 2 gene. Science. 1999; 284(5412):321-325. (Biology). 参考文献を見る
      5. Li ZW, Chu W, Hu Y, et al. The IKKbeta subunit of IkappaB kinase (IKK) is essential for nuclear factor kappaB activation and prevention of apoptosis. J Exp Med. 1999; 189(11):1839-1845. (Biology). 参考文献を見る
      6. Ling L, Cao Z, Goeddel DV. NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176. Proc Natl Acad Sci U S A. 1998; 95(7):3792-3797. (Biology). 参考文献を見る
      7. Malinin NL, Boldin MP, Kovalenko AV, Wallach D. MAP3K-related kinase involved in NF-kappaB induction by TNF, CD95 and IL-1. Nature. 1997; 385(6616):540-544. (Biology). 参考文献を見る
      8. Mercurio F, Zhu H, Murray BW, et al. IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation. Science. 1997; 278(5339):860-866. (Biology). 参考文献を見る
      9. Nakano H, Shindo M, Sakon S, et al. Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1. Proc Natl Acad Sci U S A. 1998; 95(7):3537-3542. (Biology). 参考文献を見る
      10. Rothwarf DM, Zandi E, Natoli G, Karin M. IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex. Nature. 1998; 395(6699):297-300. (Biology). 参考文献を見る
      11. Tanaka M, Fuentes ME, Yamaguchi K, et al. Embryonic lethality, liver degeneration, and impaired NF-kappa B activation in IKK-beta-deficient mice. Immunity. 1999; 10(4):421-429. (Biology). 参考文献を見る
      すべて表示する (11) 表示項目を減らす
      551920 Rev. 7

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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