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推奨アッセイ手順
Put all BD® AbSeq Reagents to be pooled into a Latch Rack for 500 µL Tubes (Thermo Fisher Scientific Cat. No. 4900). Arrange the tubes so that they can be easily uncapped and re-capped with an 8-Channel Screw Cap Tube Capper (Thermo Fisher Scientific Cat. No. 4105MAT) and the reagents aliquoted with a multi-channel pipette.
BD® AbSeq tubes should be centrifuged for ≥ 30 seconds at 400 × g to ensure removal of any content in the cap/tube threads prior to the first opening.
製品通知
- This reagent has been pre-diluted for use at the recommended volume per test. Typical use is 2 µl for 1 × 10^6 cells in a 200-µl staining reaction.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Illumina is a trademark of Illumina, Inc.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to bd.com/genomics-resources for technical protocols.
- For U.S. patents that may apply, see bd.com/patents.
コンパニオン製品
The MD22 monoclonal antibody specifically recognizes CD64 which is also known as Fc-gamma receptor I (FcgR1) or FcγRI. CD64 is a ~72 kDa type I transmembrane glycoprotein that is encoded by FCGR1A (Fc fragment of IgG receptor Ia). CD64 functions as a high affinity receptor for human IgG. The CD64 antigen is expressed on monocytes, macrophages, at low levels on polymorphonuclear neutrophils (PMNs), and on a subpopulation of circulating dendritic cells. The CD64 antigen is an early granulomonocytic lineage marker on CD34+ hematopoietic progenitors. Expression of the CD64 antigen increases transiently in cases of sepsis. The CD64 antigen functions in both innate and adaptive immune responses, and mediates endocytosis, phagocytosis, antigen presentation, antibody-dependent cellular cytotoxicity, cytokine release, and superoxide generation. The CD64 antigen associates with the signal-transducing γ-chain homodimer of Fc receptors to form the functional high affinity FcγRI complex. Ligation of the CD64 antigen leads to the activation of the protein tyrosine kinases, hck and lyn.
開発者向け参考資料 (10)
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Ernst LK, Duchemin AM, Anderson CL. Association of the high-affinity receptor for IgG (Fc gamma RI) with the gamma subunit of the IgE receptor.. Proc Natl Acad Sci USA. 1993; 90(13):6023-7. (Biology). 参考文献を見る
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Fanger NA, Wardwell K, Shen L, Tedder TF, Guyre PM. Type I (CD64) and type II (CD32) Fc gamma receptor-mediated phagocytosis by human blood dendritic cells.. J Immunol. 1996; 157(2):541-8. (Biology). 参考文献を見る
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Fischer G, Schneider EM, L Moldawer LL, et al. CD64 surface expression on neutrophils is transiently upregulated in patients with septic shock.. Intensive Care Med. 2001; 27(12):1848-52. (Biology). 参考文献を見る
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Olweus J, Terstappen LW, Thompson PA, Lund-Johansen F. Expression and function of receptors for stem cell factor and erythropoietin during lineage commitment of human hematopoietic progenitor cells. Blood. 1996; 88:1594-1607. (Biology). 参考文献を見る
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Qureshi SS, Lewis SM, Gant VA, Treacher D, Davis BH, Brown KA. Increased distribution and expression of CD64 on blood polymorphonuclear cells from patients with the systemic inflammatory response syndrome (SIRS).. Clin Exp Immunol. 2001; 125(2):258-65. (Biology). 参考文献を見る
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Scholl PR, Geha RS. Physical association between the high-affinity IgG receptor (Fc gamma RI) and the gamma subunit of the high-affinity IgE receptor (Fc epsilon RI gamma).. Proc Natl Acad Sci USA. 1993; 90(19):8847-50. (Biology). 参考文献を見る
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Wang AV, Scholl PR, Geha RS. Physical and functional association of the high affinity immunoglobulin G receptor (Fc gamma RI) with the kinases Hck and Lyn.. J Exp Med. 1994; 180(3):1165-70. (Biology). 参考文献を見る
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Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
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van de Winkel JG, Anderson CL. Biology of human immunoglobulin G Fc receptors.. J Leukoc Biol. 1991; 49(5):511-24. (Biology). 参考文献を見る
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van de Winkel JG, Capel PJ. Human IgG Fc receptor heterogeneity: molecular aspects and clinical implications.. Immunol Today. 1993; 14(5):215-21. (Biology). 参考文献を見る
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