Oligo Mouse Anti-Human CD34

BD™ AbSeq Oligo Mouse Anti-Human CD34

Name: Oligo Mouse Anti-Human CD34
Brand: Oligo Mouse Anti-Human CD34
SKU: 940367

クローン 563 (RUO)

Protocol Library

Scientific Resources

プロトコールライブラリー

科学的リソース

カタログ番号:345120

希望小売価格:¥58,000

容量:EA (1 Each)

サイズ:

25 Tests

数量:

見積に追加

見積リストに保存バルク試薬見積の依頼

製品詳細

Brand: BD™ AbSeq

Alternative Name: gp 105-120

Vol. Per Test: 2 µl

Isotype: Mouse IgG1, κ

Reactivity: Human,Rhesus,Cynomolgus (Tested in Development)

Application: Single Cell 3' Sequencing (Qualified)

Barcode Sequence: GTGTGTAGTCGCCAGTAGGTAGTTGTGTCGTAATGT

Sequence ID: AHS0191

Immunogen: CD34+ Human Leukemic Cells

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Host Species: Mouse

調製と保管

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography and conjugated to BD® AbSeq oligonucleotide under optimal conditions.

推奨アッセイ手順

Put all BD® AbSeq Reagents to be pooled into a Latch Rack for 500 µL Tubes (Thermo Fisher Scientific Cat. No. 4900). Arrange the tubes so that they can be easily uncapped and re-capped with an 8-Channel Screw Cap Tube Capper (Thermo Fisher Scientific Cat. No. 4105MAT) and the reagents aliquoted with a multi-channel pipette.

BD® AbSeq tubes should be centrifuged for ≥ 30 seconds at 400 × g to ensure removal of any content in the cap/tube threads prior to the first opening.

製品通知

This reagent has been pre-diluted for use at the recommended volume per test. Typical use is 2 µl for 1 × 10^6 cells in a 200-µl staining reaction.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
Illumina is a trademark of Illumina, Inc.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Please refer to bd.com/genomics-resources for technical protocols.
For U.S. patents that may apply, see bd.com/patents.

データシート

テクニカルデータシート（TDS）

コンパニオン製品

Human BD Fc Block™ RUO

サイズ 50 µg カタログ番号 564219

Human BD Fc Block™ RUO

サイズ 0.25 mg カタログ番号 564220

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Single-Cell Analysis System RUO

サイズ 1 EACH カタログ番号 633701

Express Single-Cell Analysis System Package RUO

サイズ 1 EACH カタログ番号 633707

Targeted mRNA and AbSeq Training Kit 4 Pack RUO

サイズ 1 Each カタログ番号 633772

詳細を表示

940367 Rev. 3

抗体の詳細

563

The 563 monoclonal antibody specifically binds to CD34. CD34 is a single-chain 105-120 kDa heavily O-glycosylated transmembrane glycoprotein expressed on hematopoietic progenitor cells, vascular endothelium and some tissue fibroblasts. The intracellular chain of the CD34 antigen is a target for phosphorylation by activated protein kinase C suggesting that CD34 may play a role in signal transduction. CD34 may play a role in adhesion of specific antigens to endothelium. Clone 563 belongs to the class III epitope; it is resistant to neuraminidase, chymopapain and glycoprotease. This antibody is able to block the binding of other CD34 mAbs like 581.

940367 Rev. 3

引用＆参考文献

View Development References

開発者向け参考資料 (11)

Egeland T, Tjonnfjord G, Steen R, Gaudernack G, Thorsby E. Positive selection of bone marrow-derived CD34 positive cells for possible stem cell transplantation. Transplant Proc. 1993; 25(1):1261-1263. (Biology). 参考文献を見る
Gaudernack G, Egeland T. Epitope mapping of 33 CD34 mAb, including the Fith Workshop Panel. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:861-864.
Greaves MF, Titley I, Colman SM, et al. CD34 cluster workshop report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:840-846.
Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
Lin G-X, Yang X, Hollemweguer E, et al. Cross-reactivity of CD antibodies in eight animal species. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:519-523.
Nishio H, Tada J, Hashiyama N, Hirn J, Ingles-Esteven J, Suda T. CD34. 1999. Available: http://mpr.nci.nih.gov/prow/guide/968267813_g.htm 2006, February 8.
Owens MA, Loken MR. Peripheral blood stem cell quantitation. In: Owens MA, Loken MR. Flow Cytometry Principles for Clinical Laboratory Practice. New York: John Wiley & Sons; 1995:128.
Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
Steen R, Egeland T. CD34 molecule epitope distribution on cells of haematopoietic origin.. Leuk Lymphoma. 1998; 30(1-2):23-30. (Clone-specific: Flow cytometry). 参考文献を見る
Strawn WB, Ferrario CM. Angiotensin II AT1 receptor blockade normalizes CD11b+ monocyte production in bone marrow of hypercholesterolemic monkeys.. Atherosclerosis. 2008; 196(2):624-32. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). 参考文献を見る

すべて表示する (11)

940367 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.