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Oligo Mouse Anti-Human CD16b
規制ステータス凡例
Becton, Dickinson and Companyの書面による明示的な許諾を得た使用以外での製品の使用は固く禁じられています。
調製と保管
推奨アッセイ手順
Put all BD® AbSeq Reagents to be pooled into a Latch Rack for 500 µL Tubes (Thermo Fisher Scientific Cat. No. 4900). Arrange the tubes so that they can be easily uncapped and re-capped with an 8-Channel Screw Cap Tube Capper (Thermo Fisher Scientific Cat. No. 4105MAT) and the reagents aliquoted with a multi-channel pipette.
BD® AbSeq tubes should be centrifuged for ≥ 30 seconds at 400 × g to ensure removal of any content in the cap/tube threads prior to the first opening.
製品通知
- This reagent has been pre-diluted for use at the recommended volume per test. Typical use is 2 µl for 1 × 10^6 cells in a 200-µl staining reaction.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Illumina is a trademark of Illumina, Inc.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to bd.com/genomics-resources for technical protocols.
- For U.S. patents that may apply, see bd.com/patents.
コンパニオン製品
Reacts with CD16b, a glycosyl phosphatidyl inositol-anchored (GPI) protein expressed on human neutrophils. Human CD16 is the low affinity Fc gamma receptor III (Fc gamma RIII) and shows two distinct forms coded by two linked genes. One form is CD16a, a polypeptide-anchored form (Fc gamma RIIIA), present on natural killer cells and macrophages. The other form is CD16b the GPI-anchored form, Fc gamma RIIIB, found on human neutrophils. CD16b is polymorphic and the two codominant alleles are referred to as Neutrophil Antigen 1 (NA1) and Neutrophil Antigen 2 (NA2). Clone CLBgran11.5 reacts with neutrophils expressing the NA1 molecule. CD16b has been reported to participate in immune complex binding by resting neutrophils and also in neutrophil transendothelial migration by interacting with integrins during the inflammation response process.
開発者向け参考資料 (4)
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Nagarajan S, Anderson M, Ahmed SN, Sell KW, Selvaraj P. Purification and optimization of functional reconstitution on the surface of leukemic cell lines of GPI-anchored Fc gamma receptor III. J Immunol Methods. 1995; 184(2):241-251. (Biology). 参考文献を見る
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Nagarajan S, Chesla S, Cobern L, Anderson P, Zhu C, Selvaraj P. Ligand binding and phagocytosis by CD16 (Fc gamma receptor III) isoforms. Phagocytic signaling by associated zeta and gamma subunits in Chinese hamster ovary cells. J Biol Chem. 1995; 270(43):25762-25770. (Biology). 参考文献を見る
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Nagarajan S, Venkiteswaran K, Anderson M, Sayed U, Zhu C, Selvaraj P. Cell-specific, activation-dependent regulation of neutrophil CD32A ligand-binding function. Blood. 2000; 95(3):1069-1077. (Biology). 参考文献を見る
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Sendo F, Suzuki K, Watanabe T, Takeda Y, Araki Y. Modulation of leukocyte transendothelial migration by integrin-associated glycosyl phosphatidyl inositol (GPI)-anchored proteins. Inflamm Res. 1998; 47(3):S133-S136. (Biology). 参考文献を見る
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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