Oligo Mouse Anti-Human CD119

BD™ AbSeq Oligo Mouse Anti-Human CD119

Name: Oligo Mouse Anti-Human CD119
Brand: Oligo Mouse Anti-Human CD119
SKU: 940253

クローン GIR-208 (RUO)

Protocol Library

Scientific Resources

プロトコールライブラリー

科学的リソース

カタログ番号:940253

希望小売価格:¥76,000

容量:EA (1 Each)

サイズ:

25 Tests

数量:

見積に追加

見積リストに保存バルク試薬見積の依頼

製品詳細

Brand: BD™ AbSeq

Alternative Name: IFN- gamma receptor alpha chain

Entrez Gene ID: 3459

Vol. Per Test: 2 µl

Isotype: Mouse IgG1, κ

Reactivity: Human (Tested in Development)

Application: Single Cell 3' Sequencing (Qualified)

Barcode Sequence: TTGTTAATGGTATGGGCGATCGAGTAGCGGAGTAGT

Sequence ID: AHS0169

Immunogen: Human IFN-γRα

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Host Species: Mouse

調製と保管

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography and conjugated to BD® AbSeq oligonucleotide under optimal conditions.

推奨アッセイ手順

Put all BD® AbSeq Reagents to be pooled into a Latch Rack for 500 µL Tubes (Thermo Fisher Scientific Cat. No. 4900). Arrange the tubes so that they can be easily uncapped and re-capped with an 8-Channel Screw Cap Tube Capper (Thermo Fisher Scientific Cat. No. 4105MAT) and the reagents aliquoted with a multi-channel pipette.

BD® AbSeq tubes should be centrifuged for ≥ 30 seconds at 400 × g to ensure removal of any content in the cap/tube threads prior to the first opening.

製品通知

This reagent has been pre-diluted for use at the recommended volume per test. Typical use is 2 µl for 1 × 10^6 cells in a 200-µl staining reaction.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
Illumina is a trademark of Illumina, Inc.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Please refer to bd.com/genomics-resources for technical protocols.
For U.S. patents that may apply, see bd.com/patents.

データシート

テクニカルデータシート（TDS）

コンパニオン製品

Human BD Fc Block™ RUO

サイズ 50 µg カタログ番号 564219

Human BD Fc Block™ RUO

サイズ 0.25 mg カタログ番号 564220

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Single-Cell Analysis System RUO

サイズ 1 EACH カタログ番号 633701

Express Single-Cell Analysis System Package RUO

サイズ 1 EACH カタログ番号 633707

Targeted mRNA and AbSeq Training Kit 4 Pack RUO

サイズ 1 Each カタログ番号 633772

詳細を表示

940253 Rev. 2

抗体の詳細

GIR-208

The GIR-208 antibody recognizes the extracellular region of CD119 which is also known as the alpha chain subunit (80-95 kDa glycoprotein) of the human interferon-γ receptor (IFN-γRα). The functionally active-form of the human IFN-γ receptor consists of two (or more) subunits, with IFN-γRα responsible for IFN-γ binding and both the IFN-γRα and β chains required for the transduction of biologic responses. The IFN-γ receptor α chain (CD119) is expressed on the surface of most human cells (except mature erythrocytes) including monocytes, macrophages, T cells, B cells, NK cells, neutrophils, fibroblasts, epithelial cells, and endothelium. Binding of 125I-labeled GIR-208 antibody to IFN-γRα+ cells is reported to be specifically inhibited in the presence of excess IFN-γ. GIR-208 does not cross react with IFN-γ as tested by ELISA. The ability of this antibody to bind to IFN-γ receptors of species other than human has not been determined. The immunogen used to generate this hybridoma was human IFN-γRα purified from human placenta. The GIR- 208 has been reported to block the binding of 125I-human IFN-γ to IFN-γRα+ cells as well as purified, soluble human IFN-γRα. GIR-208 is a neutralizing antibody that has been shown to neutralize the anti-viral activity of IFN-γ on WISH cells in a dose-dependent fashion.

940253 Rev. 2

引用＆参考文献

View Development References

開発者向け参考資料 (6)

Bach EA, Aguet M, Schreiber RD. The IFN gamma receptor: a paradigm for cytokine receptor signaling. Annu Rev Immunol. 1997; 15:563-591. (Biology). 参考文献を見る
Gumina RJ, Freire-Moar J, DeYoung L, Webb DR, Devens BH. Transduction of the IFN-gamma signal for HLA-DR expression in the promonocytic line THP-1 involves a late-acting PKC activity. Cell Immunol. 1991; 138(2):265-279. (Clone-specific: Neutralization). 参考文献を見る
Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:818-821.
Peyman JA, Hammond GL. Localization of IFN-gamma receptor in first trimester placenta to trophoblasts but lack of stimulation of HLA-DRA, -DRB, or invariant chain mRNA expression by IFN-gamma. J Immunol. 1992; 149(8):2675-2680. (Biology). 参考文献を見る
Sheehan KC, Calderon J, Schreiber RD. Generation and characterization of monoclonal antibodies specific for the human IFN-gamma receptor. J Immunol. 1988; 140(12):4231-4237. (Immunogen: Neutralization, Western blot). 参考文献を見る
Valente G, Ozmen L, Novelli F. Distribution of interferon-gamma receptor in human tissues. Eur J Immunol. 1992; 22(9):2403-2412. (Biology). 参考文献を見る

すべて表示する (6)

940253 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.