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      Purified Mouse Anti-Human c-Myc with Control

      BD Pharmingen™ Purified Mouse Anti-Human c-Myc with Control

      (RUO)
      Purified Mouse Anti-Human c-Myc with Control
      Western blot analysis of c-myc. Lysate from Jurkat cells was probed with anti-c-myc (clone 9E10, Comp. No. 51-1485GR) at concentrations of 2.0 (lane 1), 1.0 (lane 2), and 0.5 µg/ml (lane 3). C-myc is identified as a band of 62 kDa.
      Purified Mouse Anti-Human c-Myc with Control
      Western blot analysis of c-myc. Lysate from Jurkat cells was probed with anti-c-myc (clone 9E10, Comp. No. 51-1485GR) at concentrations of 2.0 (lane 1), 1.0 (lane 2), and 0.5 µg/ml (lane 3). C-myc is identified as a band of 62 kDa.
      製品詳細
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      BD Pharmingen™
      Human (QC Testing)
      Western blot (Routinely Tested), Electron microscopy, Fluorescence microscopy, Immunohistochemistry-paraffin (Reported), Immunohistochemistry-frozen (Not Recommended)
      RUO
      AB_394046


      推奨アッセイ手順

      It is particularly useful for western blot analysis of myc-tagged recombinant proteins, and recombinant c-myc. Jurkat control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (Comp. No. 51-16526N; store lysate at -20 °C). Additional control lysate is sold separately.

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      製品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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      551102 Rev. 3
      構成品
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      説明 数量/容量 Part Number EntrezGene ID
      Purified Mouse Anti-Human c-Myc 50 µg (1 ea) 51-1485GR N/A
      Jurkat Cell Lysate 50 µg (1 ea) 51-16526N N/A
      551102 Rev. 3
      引用＆参考文献
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      開発者向け参考資料 (13)

      1. Blackwood EM, Eisenman RN. Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with Myc. Science. 1991; 251(4998):1211-1217. (Biology). 参考文献を見る
      2. Campbell AM, Kessler PD, Fambrough DM. The alternative carboxyl termini of avian cardiac and brain sarcoplasmic reticulum/endoplasmic reticulum Ca(2+)-ATPases are on opposite sides of the membrane. J Biol Chem. 1992; 267(13):9321-9325. (Clone-specific: Fluorescence microscopy, Western blot). 参考文献を見る
      3. DePinho RA, Schreiber-Agus N, Alt FW. myc family oncogenes in the development of normal and neoplastic cells. Adv Cancer Res. 1991; 57:1-46. (Biology). 参考文献を見る
      4. Evan GI, Lewis GK, Ramsay G, Bishop JM. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol. 1985; 5(12):3610-3616. (Immunogen: Western blot). 参考文献を見る
      5. Gosney JR, Field JK, Gosney MA, Lye MD, Spandidos DA, Butt SA. c-myc oncoprotein in bronchial carcinoma: expression in all major morphological types. Anticancer Res. 1990; 10(3):623-628. (Clone-specific: Electron microscopy, Immunohistochemistry). 参考文献を見る
      6. Karayiannis M, Yiagnisis M, Papadimitriou K, Field JK, Spandidos DA. Evaluation of the ras and myc oncoproteins in benign gastric lesions. Anticancer Res. 1990; 10(5A):1127-1134. (Clone-specific: Immunohistochemistry). 参考文献を見る
      7. Liu ZG, Hsu H, Goeddel DV, Karin M. Dissection of TNF receptor 1 effector functions: JNK activation is not linked to apoptosis while NF-kappaB activation prevents cell death. Cell. 1996; 87(3):565-576. (Clone-specific: Western blot). 参考文献を見る
      8. Lukas J, Parry D, Aagaard L, et al. Retinoblastoma-protein-dependent cell-cycle inhibition by the tumour suppressor p16. Nature. 1995; 375(6531):503-506. (Clone-specific: Western blot). 参考文献を見る
      9. Royds JA, Sharrard RM, Wagner B, Polacarz SV. Cellular localisation of c-myc product in human colorectal epithelial neoplasia. J Pathol. 1992; 166(3):225-233. (Clone-specific: Immunohistochemistry). 参考文献を見る
      10. Spandidos DA, Yiagnisis M, Papadimitriou K, Field JK. ras, c-myc and c-erbB-2 oncoproteins in human breast cancer. Anticancer Res. 1989; 9(5):1385-1394. (Clone-specific: Immunohistochemistry). 参考文献を見る
      11. Tiniakos D, Spandidos DA, Kakkanas A, Pintzas A, Pollice L, Tiniakos G. Expression of ras and myc oncogenes in human hepatocellular carcinoma and non-neoplastic liver tissues. Anticancer Res. 1989; 9(3):715-722. (Clone-specific: Immunohistochemistry). 参考文献を見る
      12. Tosca A, Linardopoulos S, Malliri A, Hatziolou E, Nicolaidou A, Spandidos DA. Implication of the ras and myc oncoproteins in the pathogenesis of mycosis fungoides. Anticancer Res. 1991; 11(4):1433-1438. (Clone-specific: Immunohistochemistry). 参考文献を見る
      13. Williams AR, Piris J, Wyllie AH. Immunohistochemical demonstration of altered intracellular localization of the C-Myc oncogene product in human colorectal neoplasms. J Pathol. 1990; 160(4):287-293. (Clone-specific: Immunohistochemistry). 参考文献を見る
      すべて表示する (13) 表示項目を減らす
      551102 Rev. 3

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.