Skip to main content Skip to navigation
Purified Rat Anti-Mouse CD62E
Purified Rat Anti-Mouse CD62E

Immunohistochemical staining for CD62E. Frozen tongue sections of a lipopolysaccharide treated C57BL/6 mouse was reacted with the 10E9.6 antibody. Endothelial cells of the blood vessels express CD62E and can be identifed by the brown staining.

Immunohistochemical staining for CD62E. Frozen tongue sections of a lipopolysaccharide treated C57BL/6 mouse was reacted with the 10E9.6 antibody. Endothelial cells of the blood vessels express CD62E and can be identifed by the brown staining.

製品詳細
Down Arrow Up Arrow


BD Pharmingen™
Sele; E-selectin; Elam; ELAM-1; LECAM2; LYAM2
Mouse (QC Testing)
Rat LEW, also known as Lewis IgG2a, κ
Mouse brain capillary endothelioma bEnd.3 (TNFα-stimulated)
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
125 µg/ml
20339
AB_393585
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
RUO


推奨アッセイ手順

Immunohistochemistry:  The 10E9.6 antibody (Cat. No. 550290) is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. Tissue tested was tongue from LPS treated C57BL/6. The antibody stains endothelial cells in the mouse. The isotype control recommended for use with this antibody is purified rat IgG2a (Cat. No. 559073). For optimal indirect immunohistochemical staining, the 10E9.6 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with biotinylated polyclonal anti-rat Igs (multiple adsorbed) (Cat. No. 559286) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). The clone 10E9.6 is not recommended for formalin-fixed paraffin embedded sections.

A detailed protocol of the immunohistochemical procedure is available at our website, http://www.bdbiosciences.com/support/resources

製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
550290 Rev. 2
抗体の詳細
Down Arrow Up Arrow
10E9.6

The 10E9.6 monoclonal antibody specifically binds to the 97-110 kDa cell surface glycoprotein E-selectin (CD62E), also known as endothelial-leukocyte adhesion molecule-1 (ELAM-1), which is expressed on endotoxin- or cytokine-stimulated mouse endothelial cells.  A suspension of TNFα stimulated mouse brain capillary endothelioma cells, from the cell line bEnd.3, was used as the immunogen.  The epitope recognized by mAb 10E9.6 has been mapped to the first and/or second complement regulatory protein repeat domains of E-selectin. The 10E9.6 antibody has been reported to block binding of a monocyte cell line to E-selectin in vitro and to block neutrophil migration in BALB/c, but not C57BL/6 mice. It has no effect on leukocyte rolling in TNFα-treated mouse venules or on in vitro adhesion of myeloid cells to E-selectin. Studies have demonstrated that Cutaneous Lymphocyte Antigen (CLA), recognized by mAb HECA-452 (Cat. no. 555946), may be a ligand for CD62E.

550290 Rev. 2
フォーマットの詳細
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
550290 Rev.2
引用&参考文献
Down Arrow Up Arrow

開発者向け参考資料 (7)

  1. Borges E, Pendl G, Eytner R, Steegmaier M, Zollner O, Vestweber D. The binding of T cell-expressed P-selectin glycoprotein ligand-1 to E- and P-selectin is differentially regulated. J Biol Chem. 1997; 272(45):28786-28792. (Biology). 参考文献を見る
  2. Bosse R, Vestweber D. Only simultaneous blocking of the L- and P-selectin completely inhibits neutrophil migration into mouse peritoneum. Eur J Immunol. 1994; 24(12):3019-3024. (Immunogen: Blocking, ELISA, Immunoprecipitation). 参考文献を見る
  3. Eppihimer MJ, Wolitzky B, Anderson DC, Labow MA, Granger DN. Heterogeneity of expression of E- and P-selectins in vivo. Circ Res. 1996; 79(3):560-569. (Biology: Blocking). 参考文献を見る
  4. Ley K, Bullard DC, Arbones ML, et al. Sequential contribution of L- and P-selectin to leukocyte rolling in vivo. J Exp Med. 1995; 181(2):669-675. (Biology). 参考文献を見る
  5. Pendl GG, Robert C, Steinert M, et al. Immature mouse dendritic cells enter inflamed tissue, a process that requires E- and P-selectin, but not P-selectin glycoprotein ligand 1. Blood. 2002; 99(3):946-956. (Biology). 参考文献を見る
  6. Ramos CL, Kunkel EJ, Lawrence MB, et al. Differential effect of E-selectin antibodies on neutrophil rolling and recruitment to inflammatory sites. Blood. 1997; 89(8):3009-3018. (Immunogen: Blocking). 参考文献を見る
  7. Weller A, Isenmann S, Vestweber D. Cloning of the mouse endothelial selectins. Expression of both E- and P-selectin is inducible by tumor necrosis factor alpha. J Biol Chem. 1992; 267(21):15176-15183. (Biology). 参考文献を見る
すべて表示する (7) 表示項目を減らす
550290 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.