BD Pharmingen™ Purified Mouse Anti-MAP2
クローン Ap20 (RUO)
Preparation and Storage
推奨アッセイ手順
Applications include western blot analysis (1-2 µg/ml), immunoprecipitation, and immunohistochemistry of paraformaldehyde-fixed tissue cultured cells, and frozen tissue sections. T98G human glioblastoma cells (ATCC CRL-1690) and rat brain enriched microtubule protein preparations are suggested as positive controls.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Microtubule-associated protein 2 (MAP2) is a neuronal cytoskeletal protein that binds to tubulin and stabilizes microtubules. It is expressed in the cell body and dendrites of neurons, but is absent in neuronal processes. The expression of MAP2 is developmentally regulated, and there are multiple high and low molecular weight isoforms, all derived from one MAP2 gene. MAP2a, MAP2b, and MAP2c are the three major MAP2 isoforms. The high molecular weight MAP2 isoforms, MAP2a and MAP2b consist of a large projection arm and a short microtubule binding domain. The low molecular weight MAP-2 isoform, MAP2c, lacks most of the projection arm of the high molecular weight isoforms. MAP2c is the earliest expressed MAP2 and is derived by alternative splicing MAP2a and MAP2b are considered to be adult expressed MAPs, with MAP2a expression occuring later in development than MAP2b expression. MAP2a is thought to result from a post-translational modification of MAP2b. High molecular weight MAP2 isoforms (2a and 2b) migrate as a doublet at a molecular weight of >300 kDa. The low molecular weight MAP2 isoform 2c migrates at 70 kDa.
The Ap-20 antibody recognizes high molecular weight MAP2 isoforms MAP2a and MAP2b. Specifically, it has been shown to recognize MAP2a and MAP2b from human, bovine, rat, frog, and quail cells and tissues. Ap-20 does not recognize the low molecular weight MAP2 isoforms (MAP2c) or other microtubule proteins. Ap-20 reacts with an epitope between amino acids 997-1332 of high molecular weight MAP2 isoforms.4 Bovine MAP2 was used as immunogen.
Development References (4)
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Binder LI, Frankfurter A, Rebhun LI. Differential localization of MAP-2 and tau in mammalian neurons in situ. Ann N Y Acad Sci. 1986; 145-166. (Immunogen). View Reference
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Kalcheva N, Albala JS, Binder LI, Shafit-Zagardo B. Localization of specific epitopes on human microtubule-associated protein 2. J Neurochem. 1994; 63(6):2336-2341. (Clone-specific: Western blot). View Reference
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Tucker RP, Binder LI, Viereck C, Hemmings BA, Matus AI. The sequential appearance of low- and high-molecular-weight forms of MAP2 in the developing cerebellum. J Neurosci. 1988; 12:4503-4512. (Biology). View Reference
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Tucker RP. The roles of microtubule-associated proteins in brain morphogenesis: a review. Brain Res Rev. 1990; 15(2):101-120. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.