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Purified Mouse Anti-Syntaxin 8
Purified Mouse Anti-Syntaxin 8

Western blot analysis of Syntaxin 8 on human endothelial lysate.  Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Syntaxin 8.

Purified Mouse Anti-Syntaxin 8

Immunofluorescent staining of WI38 cells.

Western blot analysis of Syntaxin 8 on human endothelial lysate.  Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Syntaxin 8.

Immunofluorescent staining of WI38 cells.

製品詳細
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG2a
Mouse Syntaxin 8 aa. 57-164
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
27 kDa
250 µg/ml
AB_398875
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611352 Rev. 2
抗体の詳細
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48/Syntaxin 8

Eukaryotic protein trafficking involves the packaging of molecules into membranous vesicles that bud from a donor compartment, travel to a specific destination, fuse, and release their components into an acceptor compartment. Recognition between vesicle and acceptor membrane is mediated by the pairing of the integral membrane SNARE proteins. The stable interaction between vesicle proteins (v-SNAREs) and target proteins (t-SNAREs) juxtaposes the membranes and results in an activated docking state and/or membrane fusion. The syntaxin protein family contains a number of members that serve as functional t-SNAREs. One member of this family, syntaxin 8, has two coiled-coil domains, one in the N-terminus and one toward the C-terminus. The C-terminal domain is homologous to a similar domain in syntaxin 6 and is predicted to interact in the formation of SNARE complexes, while a hydrophobic domain in the C-terminus may be involved in membrane anchoring. Syntaxin-8 is ubiquitously expressed, but is present at high levels in the heart and localizes specifically to the ER. Thus, syntaxin-8 is a t-SNARE that is thought to be involved in the early secretory pathway of many different cell types.

611352 Rev. 2
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611352 Rev.2
引用&参考文献
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開発者向け参考資料 (2)

  1. Steegmaier M, Yang B, Yoo JS. Three novel proteins of the syntaxin/SNAP-25 family. J Biol Chem. 1998; 273(51):34171-34179. (Biology). 参考文献を見る
  2. Thoreau V, Bergès T, Callebaut I. Molecular cloning, expression analysis, and chromosomal localization of human syntaxin 8 (STX8. Biochem Biophys Res Commun. 1999; 257(2):577-583. (Biology). 参考文献を見る
611352 Rev. 2

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