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Western blot analysis of SRPK1 on HeLa cell lysate. Lane 1:1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-SRPK1.
Immunofluorescent staining of EaHy cells.
BD Transduction Laboratories™ Purified Mouse Anti-SRPK1
BD Transduction Laboratories™ Purified Mouse Anti-SRPK1
Regulatory Statusの凡例
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation and Storage
推奨アッセイ手順
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Mammalian cell pre-mRNA splicing is mediated by the spliceosome, a multi-component complex that contains two types of splicing factors: small nuclear ribonucleoprotein particles (snRNPs) and non-snRNP factors. Interactions between snRNPs and pre-mRNA ensures proper establishment of a catalytic core for the splicing reaction. However, these interactions are mediated by the non-snRNP factors. The super family of Arg/Ser-rich (RS) domain containing splicing factors are well known non-snRNPs. All of these proteins share a similar structure consisting of an N-terminal RNA recognition motif and a C-terminal RS domain. However, different SR factors have distinct specificities and function is regulated by their level of expression and by reversible phosphorylation. Two families of kinases phosphorylate SR domain-containing proteins: SR protein-specific kinases (SRPK1 and 2) and Clk/Sty. SRPL1 is specific for SR domain-containing splicing factors because it recognizes only Arg and phosphorylates only Ser. SRPK1 is expressed predominately in the pancreas, domain-containing splicing factors because it recognizes only Arg and phosphorylates only Ser. SRPK1 is expressed predominately in the pancreas, whereas SRPK2 is highly expressed in brain. SRPKs affect splice-site selection and are thought to affect alternative splicing.
Development References (2)
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Colwill K, Feng LL, Yeakley JM. SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors. J Biol Chem. 1996; 271(40):24569-24575. (Biology). View Reference
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Wang HY, Lin W, Dyck JA. SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells. J Cell Biol. 1998; 140(4):737-750. (Biology). View Reference
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