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Purified Mouse Anti-SMN
Purified Mouse Anti-SMN

Western blot analysis of SMN on a HepG2 cell lysate (Human hepatocellular carcinoma; ATCC HB-8065) (left). Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-SMN antibody.

Immunohistochemical staining of pyrimidal cells in a rat cortex, formalin-fixed paraffin-embedded tissue section, with citrate pre-treatment (magnification, 20X) (center).

Immunofluorescent staining of differentiated SH-SY5Y cells (right).  Cells were seeded in a 96 well, collagen coated imaging plate (Material # 353219) at ~ 5,000 cells per well.  Cells were incubated with 50  mM ATRA (Sigma, R2625) for 5 days, followed by 50 ng/ml BDNF (Sigma, B3795)  for 5 days.  Differentiated cells were fixed and stained using the Triton X100 fix/perm protocol (see Recommended Assay Procedure; Bioimaging protocol link) and the anti-SMN   antibody.  The second step reagent was Alexa Fluor® 488 goat anti mouse Ig (Invitrogen)(pseudo colored green). Cell nuclei were counter stained with Hoechst 33342 (pseudo colored blue). The image was taken on a BD Pathway™  855 or 435 Bioimager System using a 20x objective and merged using the BD AttoVison ™ software This antibody also stained undifferentiated SH-SY5Y, SK-N-SH, C6, U87 and U373 cells using both the Triton X100 and methanol fix/perm protocols (see Recommended Assay Procedure; Bioimaging protocol link).

Western blot analysis of SMN on a HepG2 cell lysate (Human hepatocellular carcinoma; ATCC HB-8065) (left). Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-SMN antibody.

Immunohistochemical staining of pyrimidal cells in a rat cortex, formalin-fixed paraffin-embedded tissue section, with citrate pre-treatment (magnification, 20X) (center).

Immunofluorescent staining of differentiated SH-SY5Y cells (right).  Cells were seeded in a 96 well, collagen coated imaging plate (Material # 353219) at ~ 5,000 cells per well.  Cells were incubated with 50  mM ATRA (Sigma, R2625) for 5 days, followed by 50 ng/ml BDNF (Sigma, B3795)  for 5 days.  Differentiated cells were fixed and stained using the Triton X100 fix/perm protocol (see Recommended Assay Procedure; Bioimaging protocol link) and the anti-SMN   antibody.  The second step reagent was Alexa Fluor® 488 goat anti mouse Ig (Invitrogen)(pseudo colored green). Cell nuclei were counter stained with Hoechst 33342 (pseudo colored blue). The image was taken on a BD Pathway™  855 or 435 Bioimager System using a 20x objective and merged using the BD AttoVison ™ software This antibody also stained undifferentiated SH-SY5Y, SK-N-SH, C6, U87 and U373 cells using both the Triton X100 and methanol fix/perm protocols (see Recommended Assay Procedure; Bioimaging protocol link).

製品詳細
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BD Transduction Laboratories™
Survival Motor Neuron
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Human SMN aa. 14-174
Western blot (Routinely Tested), Bioimaging, Immunofluorescence, Immunohistochemistry-formalin (antigen retrieval required) (Tested During Development)
40 kDa
250 µg/ml
AB_397973
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
  6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  7. Triton is a trademark of the Dow Chemical Company.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
610647 Rev. 2
抗体の詳細
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8/SMN

SMN (survival motor neuron) was discovered as a candidate gene, located in chromosome 5q13, for the fatal autosomal Spinal muscular atrophy (SMA) disorder.  The SMN gene was missing or interrupted in a significant number of patients with SMA.  The SMN protein is 294 amino acids and migrates with apparent molecular weight of 40 kDa.  In addition to the cytoplasm, other studies localized SMN in dots of 0.1-1.0 µm within the nucleus.  These novel nuclear structures were named "gems" and found associated to coiled bodies.  It was also found that SMN interacts with the RGG box of hnRNP U and fibrillarin.  Therefore, the biochemical function of SMN may be in the regulation of mRNA metabolism.

610647 Rev. 2
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610647 Rev.2
引用&参考文献
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開発者向け参考資料 (5)

  1. Cifuentes-Diaz C, Frugier T, Tiziano FD. Deletion of murine SMN exon 7 directed to skeletal muscle leads to severe muscular dystrophy. J Cell Biol. 2001; 152(5):1107-1114. (Biology: Western blot). 参考文献を見る
  2. Claus P, Doring F, Gringel S. Differential intranuclear localization of fibroblast growth factor-2 isoforms and specific interaction with the survival of motoneuron protein. J Biol Chem. 2003; 278(1):479-485. (Biology: Immunoprecipitation, Western blot). 参考文献を見る
  3. Côté J, Boisvert FM, Boulanger MC, Bedford MT, Richard S. Sam68 RNA binding protein is an in vivo substrate for protein arginine N-methyltransferase 1. Mol Biol Cell. 2003; 14(1):274-287. (Biology: Immunoprecipitation, Western blot). 参考文献を見る
  4. Lefebvre S, Bürglen L, Reboullet S. Identification and characterization of a spinal muscular atrophy-determining gene. Cell. 1995; 80(1):155-165. (Biology). 参考文献を見る
  5. Wang IF, Reddy NM, Shen CK. Higher order arrangement of the eukaryotic nuclear bodies. Proc Natl Acad Sci U S A. 2002; 99(21):13583-13588. (Biology: Immunofluorescence, Western blot). 参考文献を見る
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610647 Rev. 2

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.