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Purified Mouse Anti-Rab5
Purified Mouse Anti-Rab5

Western blot analysis of Rab5 on a human endothelial cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- Rab5 antibody.

Purified Mouse Anti-Rab5

Immunoflourescent staining on WI-38 cells.

Western blot analysis of Rab5 on a human endothelial cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- Rab5 antibody.

Immunoflourescent staining on WI-38 cells.

製品詳細
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BD Transduction Laboratories™
Human (QC Testing), Dog (Tested in Development)
Mouse IgG2a
Human Rab5 aa. 1-215
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development)
25 kDa
250 µg/ml
AB_398048
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610724 Rev. 1
抗体の詳細
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1/Rab5

Rab5 is a low molecular weight GTP-binding protein that plays a role in endocytic vesicle traffic. Like other Rab proteins, Rab5 has C-terminal cysteine residues that are post-translationally modified by geranylgeranylation which is critical for its membrane targeting. Rab5 is associated with early endosome and plasma membranes and evidence suggests that Rab5 regulates early endosome fusion. The GTP/GDP cycle controls shuttling of Rab proteins between the cytosol and membranes. In vitro, Rab5 proteins are removed from membranes by a GDP dissociation inhibitor protein (rabGDI) which leads to the formation of a cytosolic Rab5-rabGDI complex. Rab5 may insert into membranes by a multistep process in a which a transient GDP-Rab5 intermediate is formed and converted into GTP-Rab5 that subsequently enters the acceptor membrane and releases rabGDI into the cytosol.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610724 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610724 Rev.1
引用&参考文献
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開発者向け参考資料 (4)

  1. Li G, Stahl PD. Structure-function relationship of the small GTPase rab5. J Biol Chem. 1993; 268(32):24475-24480. (Biology). 参考文献を見る
  2. Sanford JC, Pan Y, Wessling-Resnick M. Prenylation of Rab5 is dependent on guanine nucleotide binding. J Biol Chem. 1993; 268(32):23773-23776. (Biology). 参考文献を見る
  3. Stenmark H, Vitale G, Ullrich O, Zerial M. Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion. Cell. 1995; 83(3):423-432. (Biology). 参考文献を見る
  4. Ullrich O, Horiuchi H, Bucci C, Zerial M. Membrane association of Rab5 mediated by GDP-dissociation inhibitor and accompanied by GDP/GTP exchange. Nature. 1994; 368(6467):157-160. (Biology). 参考文献を見る
すべて表示する (4) 表示項目を減らす
610724 Rev. 1

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