Preparation and Storage
推奨アッセイ手順
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
関連製品
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Cellular morphology, adhesion, and motility occur through the reorganization of the actin cytoskeleton. This reorganization of actin filaments occurs through the interactions between actin and actin binding proteins. Actin-binding proteins regulate the polymerization and depolymerization of actin, connect actin-based structures to membranes and to other cytoskeletal elements, power the movement of actin filaments, and cross-link actin filaments into bundles. Actin related proteins (Arp) 2/3 complex is an actin polymerization inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20 Arc, and p16-Arc. The Arp2 and Arp3 subunits may nucleate actin polymerization, while the p41-Arc subunit is a WD repeat-containing protein that may regulate both the activity and localization of the Arp2/3 complex. Arp3, p34-Arc, and p21-Arc are localized to the lamellipodia of stationary and locomoting fibroblasts. Both WASP and Abp1p are acidic sequence-containing proteins that activate the Arp2/3 complex. However, WASP binds actin monomers, while the endocytosis-related Abp1p protein binds actin filaments. Thus, Arp2/3 complex may regulate actin polymerization in specific cell locations through interaction with actin binding Arp2/3 activators.
Development References (2)
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Welch MD, DePace AH, Verma S, Iwamatsu A, Mitchison TJ. The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. J Cell Biol. 1997; 138(2):375-384. (Biology). View Reference
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Zhao X, Yang Z, Qian M, Zhu X. Interactions among subunits of human Arp2/3 complex: p20-Arc as the hub. Biochem Biophys Res Commun. 2001; 280(2):513-517. (Biology). View Reference
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