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Western blot analysis of NuMA on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- NuMA antibody. NuMA has been reported to have a calculated molecular weight of 238 kDa, but may be observed to be migrating in a range between 238-290 kDa in HeLa whole cell extracts.
Immunofluorescence staining for NuMA in rabbit kidney.
BD Transduction Laboratories™ Purified Mouse Anti- NuMA
BD Transduction Laboratories™ Purified Mouse Anti- NuMA
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Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation and Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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関連製品
NuMA (Nuclear Mitotic Apparatus protein) is a 2115 amino acid protein with a coiled-coil structure similar to that of myosins and intermediate filaments. Indirect immunofluorescence assays indicate that NuMA's localization is very dynamic. During interphase, NuMA is in the nucleus and during mitosis it moves to the polar regions of the mitotic spindle. NuMA is a very abundant phosphoprotein and antibodies to this protein are often found in patients with autoimmune diseases. Although NuMA is thought to be a structural component of the nucleus, the precise cellular function for this protein is still unknown.
Development References (4)
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Elbi C, Misteli T, Hager GL. Recruitment of dioxin receptor to active transcription sites. Mol Biol Cell. 2002; 13(6):2001-2015. (Biology: Immunofluorescence). View Reference
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Munnia A, Schutz N, Romeike BF, et al. Expression, cellular distribution and protein binding of the glioma amplified sequence (GAS41), a highly conserved putative transcription factor. Oncogene. 2001; 20(35):4853-4863. (Biology: Immunofluorescence). View Reference
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Steen RL, Cubizolles F, Le Guellec K, Collas P. A kinase-anchoring protein (AKAP)95 recruits human chromosome-associated protein (hCAP)-D2/Eg7 for chromosome condensation in mitotic extract. J Cell Biol. 2000; 149(3):531-536. (Biology: Immunofluorescence). View Reference
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Yang CH, Lambie EJ, Snyder M. NuMA: an unusually long coiled-coil related protein in the mammalian nucleus. J Cell Biol. 1992; 116(6):1303-1317. (Biology). View Reference
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