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Purified Mouse Anti-MSH6
Purified Mouse Anti-MSH6

Western blot analysis of MSH6/GTBP on A431 lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-MSH6/GTBP.

Purified Mouse Anti-MSH6

Immunofluorescent staining of C3H10T1/2 cells.

Western blot analysis of MSH6/GTBP on A431 lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-MSH6/GTBP.

Immunofluorescent staining of C3H10T1/2 cells.

製品詳細
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BD Transduction Laboratories™
GTBP
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Human MSH6 aa. 225-333
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
160 kDa
250 µg/ml
AB_398234
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610918 Rev. 1
抗体の詳細
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44/MSH6

DNA mismatch repair in bacteria is carried out by the MutL, MutH, and MutS proteins. Initial binding of MutS to the mismatched DNA is followed by binding of the MutH endonuclease and MutL. Together these proteins form a complex that mediates excision repair. Mutations or deficiencies of any of these bacterial proteins results in a mutator phenotype that is characterized by genetic instability. MSH2, MSH3, and MSH6 are human homologs of MutS, while MLH1, PMS1, and PMS2 are homologs of MutL. As a heterodimer with MSH2, MSH6 binds to DNA containing G/T mismatches. The MSH2-MSH6 complex recognizes single-base mispairs and insertion/deletion loops. Binding of this complex induces conformational changes in the DNA that lead to the binding of an MLH-PMS1 complex and excision repair. Mutations in the human genes are associated with hereditary nonpolyposis colon cancer (HNPCC), a common hereditary disease in humans. HNPCC is characterized by frequent microsatellite mutations that arise from somatic mutation due to a replication error (RER+) phenotype. This phenotype is analogous to the bacterial system and is directly linked to DNA mismatch repair deficiencies.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or  reported in the literature.

610918 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610918 Rev.1
引用&参考文献
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開発者向け参考資料 (5)

  1. Christmann M, Kaina B. Nuclear translocation of mismatch repair proteins MSH2 and MSH6 as a response of cells to alkylating agents. J Biol Chem. 2000; 275(46):36256-36262. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). 参考文献を見る
  2. Humbert O, Hermine T, Hernandez H, et al. Implication of protein kinase C in the regulation of DNA mismatch repair protein expression and function. J Biol Chem. 2002; 277(20):18061-18068. (Clone-specific: Gel shift, Western blot). 参考文献を見る
  3. Kariola R, Otway R, Lonnqvist KE, et al. Two mismatch repair gene mutations found in a colon cancer patient--which one is pathogenic. Hum Genet. 2003; 112(2):105-109. (Clone-specific: Immunohistochemistry, Immunoprecipitation, Western blot). 参考文献を見る
  4. Palombo F, Gallinari P, Iaccarino I, et al. GTBP, a 160-kilodalton protein essential for mismatch-binding activity in human cells. Science. 1995; 268(5219):1912-1914. (Biology). 参考文献を見る
  5. Saitoh H, Pizzi MD, Wang J. Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358. J Biol Chem. 2002; 277(7):4755-4763. (Clone-specific: Immunofluorescence). 参考文献を見る
すべて表示する (5) 表示項目を減らす
610918 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.