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Purified Mouse Anti-mGluR1
Purified Mouse Anti-mGluR1

Western blot analysis of mGluR1 on a rat cerebrum lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the mouse anti-mGluR1 antibody.

Purified Mouse Anti-mGluR1

Immunofluorescence staining of cortical neurons.

Western blot analysis of mGluR1 on a rat cerebrum lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the mouse anti-mGluR1 antibody.

Immunofluorescence staining of cortical neurons.

製品詳細
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BD Transduction Laboratories™
Metabotropic Glutamate Receptor-1
Rat (QC Testing), Mouse (Tested in Development)
Mouse IgG1
Rat mGluR1 aa. 1042-1160
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
133 kDa
250 µg/ml
AB_398278
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
抗体の詳細
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20/mGluR1

Glutamate is a major excitatory neurotransmitter and functions in multiple roles in the CNS.  The functional diversity of glutamate is exemplified by two distinct groups of glutamate receptors: ionotropic and metabotropic.  Coupling with G proteins provides the metabotropic glutamate receptors (mGluRs) with the capacity for intracellular signal transduction.  Eight metabotropic glutamate receptors (mGluR1-8) and several Ca[2+] sensing receptors belong to a novel G-protein coupled receptor (GPCR) family.  The mGluRs possess the seven putative transmembrane domains which are characteristic of GPCR proteins.  However, they exhibit no additional sequence homology to any member of other GPCR families.  mGluR1 has large hydrophilic sequences in both the N- and C-terminal sides of the seven transmembrane domains.  The sizable extracellular N-terminal domain is homologous to bacterial periplasmic binding proteins and serves as the glutamate binding site.  mGluR1 activates phospholipase C (PLC), resulting in phosphoinositide turnover and, in turn, Ca2+ mobilization necessary for many signal transduction events.

フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
引用&参考文献
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開発者向け参考資料 (5)

  1. Gomeza J, Joly C, Kuhn R, Knopfel T, Bockaert J, Pin JP. The second intracellular loop of metabotropic glutamate receptor 1 cooperates with the other intracellular domains to control coupling to G-proteins. J Biol Chem. 1996; 271(4):2199-2205. (Biology). 参考文献を見る
  2. Kryl D, Yacoubian T, Haapasalo A, Castren E, Lo D, Barker PA. Subcellular localization of full-length and truncated Trk receptor isoforms in polarized neurons and epithelial cells. J Neurosci. 1999; 19(14):5823-5833. (Biology: Western blot). 参考文献を見る
  3. Mary S, Gomeza J, Prezeau L, Bockaert J, Pin JP. A cluster of basic residues in the carboxyl-terminal tail of the short metabotropic glutamate receptor 1 variants impairs their coupling to phospholipase C. J Biol Chem. 1998; 273(1):425-432. (Biology). 参考文献を見る
  4. Masu M, Tanabe Y, Tsuchida K, Shigemoto R, Nakanishi S. Sequence and expression of a metabotropic glutamate receptor. Nature. 1991; 349(6312):760-765. (Biology). 参考文献を見る
  5. Tanabe Y, Masu M, Ishii T, Shigemoto R, Nakanishi S. A family of metabotropic glutamate receptors. Neuron. 1992; 8(1):169-179. (Biology). 参考文献を見る
すべて表示する (5) 表示項目を減らす

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