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Purified Mouse Anti-IKKγ
Purified Mouse Anti-IKKγ

Western blot analysis of IKKγ on rat kidney lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-IKKγ antibody.

Purified Mouse Anti-IKKγ

Immunofluorescent staining of ES2 cells with anti-IKKγ antibody.

Western blot analysis of IKKγ on rat kidney lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-IKKγ antibody.

Immunofluorescent staining of ES2 cells with anti-IKKγ antibody.

製品詳細
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BD Transduction Laboratories™
NEMO
Rat (QC Testing), Human, Dog (Tested in Development)
Mouse IgG1
Mouse IKKγ/NEMO aa. 278-396
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
48 kDa
250 µg/ml
AB_398832
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611306 Rev. 1
抗体の詳細
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54/IKKγ/NEMO

In most cells, NF-κB is sequestered in an inactive cytoplasmic form via interactions with the inhibitory proteins IκBα, IκBβ, and IκBε. Cell stimulation induces the release, activation, and nuclear translocation of NF-κB. Release of NF-κB results from the phosphorylation and subsequent proteolytic degradation of the IκB proteins. Two cytokine-inducible IκB kinases (IKKα and IKKβ) phosphorylate and target the IκB proteins for degradation by the ubiquitin pathway. These kinases are components of a 700-900 kDa multisubunit complex that also contains NF-κB/RelA, IκBα, MEKK1, NIK, IKAP, and IKKγ/NEMO (NF-κB essential modulator). IKKγ contains two coiled-coil domains and a leucine zipper which allow it to form dimers and trimers that interact directly with IKKβ. IKKγ, essential for IKKα/IKKβ activation of NF-κB, is located functionally and physically upstream of these subunits. In addition, IKKγ enables the HTLV-1 Tax oncoprotein to interact with IKKβ, resulting in constitutive activation of NF-κB and maintenance of cellular transformation. Thus, IKKγ is an essential element of the IκB complex and an adaptor that mediates stable formation of Tax-IKK complexes.

611306 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611306 Rev.1
引用&参考文献
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開発者向け参考資料 (5)

  1. Chu ZL, Shin YA, Yang JM, DiDonato JA, Ballard DW. IKKgamma mediates the interaction of cellular IkappaB kinases with the tax transforming protein of human T cell leukemia virus type 1. J Biol Chem. 1999; 274(22):15297-15300. (Biology). 参考文献を見る
  2. Jin DY, Giordano V, Kibler KV, Nakano H, Jeang KT. Role of adapter function in oncoprotein-mediated activation of NF-kappaB. Human T-cell leukemia virus type I Tax interacts directly with IkappaB kinase gamma. J Biol Chem. 1999; 274(25):17402-17405. (Biology). 参考文献を見る
  3. Rothwarf DM, Zandi E, Natoli G, Karin M. IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex. Nature. 1998; 395(6699):297-300. (Biology). 参考文献を見る
  4. Weil R, Schwamborn K, Alcover A, Bessia C, Di Bartolo V, Israël A. Induction of the NF-kappaB cascade by recruitment of the scaffold molecule NEMO to the T cell receptor. Immunity. 2003; 18(1):13-26. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). 参考文献を見る
  5. Yamaoka S, Courtois G, Bessia C. Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation. Cell. 1998; 93(7):1231-1240. (Biology). 参考文献を見る
すべて表示する (5) 表示項目を減らす
611306 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.