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Western blot analysis of XRCC4 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human XRCC4 antibody.
Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).
BD Transduction Laboratories™ Purified Mouse Anti-Human XRCC4
BD Transduction Laboratories™ Purified Mouse Anti-Human XRCC4
Regulatory Statusの凡例
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation and Storage
推奨アッセイ手順
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
関連製品
DNA double-strand breaks (DSB) are generated during intrinsic eukaryotic DNA recombination events such as assembly of antigen receptor genes and meiotic and mitotic recombination. DSB repair proteins are also required to repair breaks induced by extrinsic factors such as ionizing radiation and mutagenic chemicals. DNA-PKcs, Ku70/Ku80, DNA ligase IV, and X-Ray Cross Complementation group 4 (XRCC4) are DSB proteins involved in both V(D)J recombination and DNA double-stranded break repair. XRCC4 activates DNA ligase IV and cells deficient in XRCC4 inefficiently form coding joints and signal joints during V(D)J recombination. XRCC4 contains a C-terminal nuclear localization sequence (NLS) and multiple phosphorylation sites, binds DNA, and is an effective substrate for DNA-PK. Phosphorylation of XRCC4 has no effect on its interactions with DNA ligase IV or end-joining activity, but can inhibit its DNA binding activity. Mice deficient in XRCC4 exhibit defects in lymphogenesis and apoptotic death of postmitotic neurons during neurogenesis. Thus, XRCC4 is a ubiquitous protein involved in DNA end joining during DNA recombination and repair, which is critical for cell growth and survival.
Development References (3)
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Gao Y, Sun Y, Frank KM, et al. A critical role for DNA end-joining proteins in both lymphogenesis and neurogenesis. Cell. 1998; 95(7):891-902. (Biology). View Reference
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Li Z, Otevrel T, Gao Y, et al. The XRCC4 gene encodes a novel protein involved in DNA double-strand break repair and V(D)J recombination. Cell. 1995; 83(7):1079-1089. (Biology). View Reference
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Modesti M, Hesse JE, Gellert M. DNA binding of Xrcc4 protein is associated with V(D)J recombination but not with stimulation of DNA ligase IV activity. EMBO J. 1999; 18(7):2008-2018. (Biology). View Reference
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