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Purified Mouse Anti-Human p230 trans Golgi
Purified Mouse Anti-Human p230 trans Golgi

Western blot analysis of p230 trans-Golgi on a HeLa lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- p230 trans Golgi antibody.

Purified Mouse Anti-Human p230 trans Golgi

Immunoflourescence staining of HeLa cells.

Western blot analysis of p230 trans-Golgi on a HeLa lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- p230 trans Golgi antibody.

Immunoflourescence staining of HeLa cells.

製品詳細
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human p230 trans Golgi aa. 2063-2179
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
230 kDa
250 µg/ml
AB_398809
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611280 Rev. 1
抗体の詳細
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15/p230 trans Golgi

The Golgi apparatus is a very complex and dynamic organelle that functions in protein sorting and modification. Numerous structural and regulatory proteins are involved in the budding, docking, and fusion of Golgi-directed vesicles. p230 trans Golgi is a peripheral membrane protein associated with the cytosolic face of the trans Golgi network (TGN). Protein sequence analysis indicates that it is highly hydrophilic and contains two proline-rich regions, as well as a high frequency of heptad repeats which are characteristic of α-helices that form dimeric coiled-coiled structures. Additionally, p230 trans Golgi contains a ESLALEELEL sequence, a motif of the acidic granin proteins that are found in secretory granules of neuroendocrine cells. p230 trans Golgi cycles between the cytosol and the TGN via non-clathrin coated vesicles in a G protein-dependent manner. The localization of p230 trans Golgi to the TGN is dependent on aromatic residues in its C-terminal non-coiled coil domain. Thus, p230 trans-Golgi is aTGN-specific protein that is important for the biogenesis of distinct populations of non-clathrin coated vesicles and, thus, functions in vesicular transport from the TGN.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611280 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611280 Rev.1
引用&参考文献
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開発者向け参考資料 (3)

  1. Erlich R, Gleeson PA, Campbell P, Dietzsch E, Toh BH. Molecular characterization of trans-Golgi p230. A human peripheral membrane protein encoded by a gene on chromosome 6p12-22 contains extensive coiled-coil alpha-helical domains and a granin motif. J Biol Chem. 1996; 271(14):8328-8337. (Biology). 参考文献を見る
  2. Kjer-Nielsen L, Teasdale RD, van Vliet C, Gleeson PA. A novel Golgi-localisation domain shared by a class of coiled-coil peripheral membrane proteins. Curr Biol. 1999; 9(7):385-388. (Biology). 参考文献を見る
  3. Kjer-Nielsen L, van Vliet C, Erlich R, Toh BH, Gleeson PA. The Golgi-targeting sequence of the peripheral membrane protein p230. J Cell Sci. 1999; 112(1):1645-1654. (Biology). 参考文献を見る
611280 Rev. 1

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