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Western blot analysis of Nup88 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the Mouse Anti-Human Nup88 antibody.
Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).
BD Transduction Laboratories™ Purified Mouse Anti-Human Nup88
BD Transduction Laboratories™ Purified Mouse Anti-Human Nup88
Regulatory Statusの凡例
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation and Storage
推奨アッセイ手順
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
Nucleocytoplasmic transport involves the binding and interaction of several cytosolic and nuclear pore proteins by at least two different mechanisms. One mechanism is mediated by karyopherins to transport proteins with the nuclear localization sequence (NLS) and an alternative pathway uses the protein Transportin. Morphologically both pathways require the use of the nuclear pore complex (NPC) which acts as a gate mediating active transport of proteins and RNA into and out of the nucleus. The NLS binds to karyopherin α, and to the N-terminal region of karyopherin β. Both karyopherins bind to repeat sequences of nucleoporins at the nuclear envelope. p62 is the best characterized member of a group of nucleoporins that line the central region of the NPC. A tightly associated complex is formed by p62 and two other nucleoporins, p54 and p58. Nup88 is a nonglycosylated nucleoporin, that does not contain the characteristic GLFG or XFXFG repeats found in other NPC proteins. The structure of Nup88 includes a coiled-coil domain required for association with the NPC. Nup88 is overexpressed during oncogenesis and development, and forms a complex with the nuclear transport factors, Exportin-1 and Nup214.
Development References (3)
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Bastos R, Ribas de Pouplana L, Enarson M, Bodoor K, Burke B. Nup84, a novel nucleoporin that is associated with CAN/Nup214 on the cytoplasmic face of the nuclear pore complex. J Cell Biol. 1997; 137(5):989-1000. (Biology). View Reference
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Fornerod M, van Deursen J, van Baal S. The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88. EMBO J. 1997; 16(4):807-816. (Biology). View Reference
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Martinez N, Alonso A, Moragues MD, Ponton J, Schneider J. The nuclear pore complex protein Nup88 is overexpressed in tumor cells. Cancer Res. 1999; 59(21):5408-5411. (Biology). View Reference
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