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Purified Mouse Anti-Human NPAT
Purified Mouse Anti-Human NPAT

Western blot analysis of NPAT on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:100, lane 2: 1:200, lane 3: 1:400 dilution of the mouse anti-human NPAT antibody.

Purified Mouse Anti-Human NPAT

Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

Western blot analysis of NPAT on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:100, lane 2: 1:200, lane 3: 1:400 dilution of the mouse anti-human NPAT antibody.

Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

製品詳細
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BD Transduction Laboratories™
Nuclear Protein Ataxia Telangiectasia
Human (QC Testing)
Mouse IgG2b
Human NPAT aa. 681-803
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
210 kDa
250 µg/ml
AB_398866
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611344 Rev. 1
抗体の詳細
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27/NPAT

Cyclins regulate transitions between cell cycle phases by acting as regulatory subunits of the cyclin-dependent kinases (cdk). The temporal expression of cyclins is tightly regulated and plays a critical role in controlling the enzymatic activity of the cdks. Cyclin-dependent kinase 2 (Cdk2) is a member of a family of cdc2-related cell cycle protein kinases. Cdk2 is expressed earlier in the cell cycle than cdc2 and forms complexes with cyclins A, E, D1, and D3. It is not known if the D cyclins can form active complexes with Cdk2. Cyclin E-Cdk2 kinase is active in the G1 and S phases of the cell cycle and is important, as is Cyclin A-Cdk2, for the progression from G1 to S phase. One substrate for cyclin E-Cdk2 is a Nuclear Protein mapped to the Ataxia Telangiectasia locus, NPAT. This protein associates with cyclin E-Cdk2 and can be phosphorylated by Cdk2. NPAT  protein levels peak at the G1/S boundary and overexpression of NPAT accelerates S phase entry, especially after coexpression of cyclin E-Cdk2. Thus, NPAT is a substrate of cyclin E-Cdk2 that may mediate G1 to S phase transition.

611344 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611344 Rev.1
引用&参考文献
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開発者向け参考資料 (1)

  1. Zhao J, Dynlacht B, Imai T, Hori T, Harlow E. Expression of NPAT, a novel substrate of cyclin E-CDK2, promotes S-phase entry. Genes Dev. 1998; 12(4):456-461. (Biology). 参考文献を見る
611344 Rev. 1

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