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Purified Mouse Anti-Human DEK
Purified Mouse Anti-Human DEK

Western blot analysis of DEK on a Jurkat cell lysate. 1:500 (lane 1), 1:1000 (lane 2), 1:2000 (lane 3) dilution of the anti- human DEK antibody.

Purified Mouse Anti-Human DEK

Immunofluoresence staining on 293 cells.

Western blot analysis of DEK on a Jurkat cell lysate. 1:500 (lane 1), 1:1000 (lane 2), 1:2000 (lane 3) dilution of the anti- human DEK antibody.

Immunofluoresence staining on 293 cells.

製品詳細
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human DEK aa. 19-169
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
50 kDa
250 µg/ml
AB_398261
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610948 Rev. 1
抗体の詳細
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2/DEK

The (6;9) chromosomal translocation is associated with acute myelogenousleukemia (AML) and fuses the dek and can genes. This results in expression of the oncogenic DEK-CAN fusion protein, consisting of the N-terminal two-thirds of DEK and the C-terminal two-thirds of CAN. Although, on its own, DEK exhibits anti-oncogenic properties, the DEK-CAN chimera appears to be oncogenic. DEK is a nuclear protein with a calculated molecular weight of 42-43 kD, that can be observable at 50 kD, and reportedly exhibits no substantial homology to any known protein sequences. Although it contains 42% charged amino acids and multiple acidic sequences, specific structural features have yet to be identified. In addition to its involvement in AML, DEK is associated with several disease states, such as juvenile rheumatoid arthritis where it is an autoantigen. Efforts to define the cellular function of DEK led to its identification as the pets factor. The peri-ets (pets) site is a TG-rich element between the two Elf-1 binding sites of the HIV-2 enhancer. The pets site mediates transcriptional activation in response to T cell stimulation. Thus, DEK is a site-specific DNA binding protein that functions in transcriptional regulation and signal transduction.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610948 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610948 Rev.1
引用&参考文献
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開発者向け参考資料 (3)

  1. Fu GK, Grosveld G, Markovitz DM. DEK, an autoantigen involved in a chromosomal translocation in acute myelogenous leukemia, binds to the HIV-2 enhancer. Proc Natl Acad Sci U S A. 1997; 94(5):1811-1815. (Biology). 参考文献を見る
  2. Fu GK, Markovitz DM. Purification of the pets factor. A nuclear protein that binds to the inducible TG-rich element of the human immunodeficiency virus type 2 enhancer. J Biol Chem. 1996; 271(32):19599-19605. (Biology). 参考文献を見る
  3. von Lindern M, Fornerod M, van Baal S, et al. The translocation (6;9), associated with a specific subtype of acute myeloid leukemia, results in the fusion of two genes, dek and can, and the expression of a chimeric, leukemia-specific dek-can mRNA. Mol Cell Biol. 1992; 12(4):1687-1697. (Biology). 参考文献を見る
610948 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.