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Purified Mouse Anti-Glutamine Synthetase
Purified Mouse Anti-Glutamine Synthetase

Western blot analysis of glutamine synthetase on a rat cerebrum lysate (left). Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the anti- glutamine synthetase antibody.

Glutamine synthetase staining on a rat cerebrum section (center). Section prepared during antibody development was formalin fixed and paraffin embedded without citrate buffer pretreatment. Note visible staining of astrocytes in the section. Magnification: 40X.

Immunofluorescent staining of SK-N-SH cells (right).  Cells were seeded in a 384 well collagen coated Microplates (Material # 353962) at ~ 8,000 cells per well.  After overnight incubation, cells were stained using the methanol fix/perm protocol (see Recommended Assay Procedure; Bioimaging protocol link) and the anti- Glutamine Synthetase antibody.  The second step reagent was Alexa Fluor® 488 goat anti mouse Ig (Invitrogen)(pseudo colored green). Cell nuclei were counter stained with Hoechst 33342 (pseudo colored blue). The image was taken on a BD Pathway™  855 or 435 Bioimager System using a 20x objective and merged using the BD AttoVison ™ software.  This antibody also stained SH-SY5Y, C6, U87 and U373 cells using both the Triton™ X-100 and methanol fix/perm protocols (see Recommended Assay Procedure; Bioimaging protocol link).

Western blot analysis of glutamine synthetase on a rat cerebrum lysate (left). Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the anti- glutamine synthetase antibody.

Glutamine synthetase staining on a rat cerebrum section (center). Section prepared during antibody development was formalin fixed and paraffin embedded without citrate buffer pretreatment. Note visible staining of astrocytes in the section. Magnification: 40X.

Immunofluorescent staining of SK-N-SH cells (right).  Cells were seeded in a 384 well collagen coated Microplates (Material # 353962) at ~ 8,000 cells per well.  After overnight incubation, cells were stained using the methanol fix/perm protocol (see Recommended Assay Procedure; Bioimaging protocol link) and the anti- Glutamine Synthetase antibody.  The second step reagent was Alexa Fluor® 488 goat anti mouse Ig (Invitrogen)(pseudo colored green). Cell nuclei were counter stained with Hoechst 33342 (pseudo colored blue). The image was taken on a BD Pathway™  855 or 435 Bioimager System using a 20x objective and merged using the BD AttoVison ™ software.  This antibody also stained SH-SY5Y, C6, U87 and U373 cells using both the Triton™ X-100 and methanol fix/perm protocols (see Recommended Assay Procedure; Bioimaging protocol link).

製品詳細
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BD Transduction Laboratories™
Glutamate-Ammonia Ligase; GLUL; GLNS
Rat (QC Testing), Human, Mouse (Tested in Development)
Mouse IgG2a
Human Glutamine Synthetase aa. 1-373
Western blot (Routinely Tested), Bioimaging, Immunohistochemistry (Tested During Development), Immunofluorescence, Immunoprecipitation (Not Recommended)
45 kDa
250 µg/ml
AB_397879
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


推奨アッセイ手順

For Western blot and IHC: Please refer to http://www.bdbiosciences.com/resources/cellbiology/index.jsp

For Bioimaging: Please refer to http://www.bdbiosciences.com/resources/cellularimaging/index.jsp

製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. Triton is a trademark of the Dow Chemical Company.
610517 Rev. 2
抗体の詳細
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6/Glutamine Synthetase

Glutamine synthetase catalyzes the amination of glutamic acid to form glutamine. It is found in mammals as an octamer of identical 45 kDa subunits. Glutamine synthetase activity is a useful marker for astrocytes and an important differentiation feature in retina. It is also considered to be a key enzyme in the recycling of the neurotransmitter glutamate.

610517 Rev. 2
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610517 Rev.2
引用&参考文献
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開発者向け参考資料 (5)

  1. Fei ZL, D'Ambrosio C, Li S, Surmacz E, Baserga R. Association of insulin receptor substrate 1 with simian virus 40 large T antigen. Mol Cell Biol. 1995; 15(8):4232-4239. (Biology: Immunoprecipitation). 参考文献を見る
  2. Kentroti S, Baker R, Lee K, Bruce C, Vernadakis A. Platelet-activating factor increases glutamine synthetase activity in early and late passage C-6 glioma cells. J Neurosci Res. 1991; 28(4):497-506. (Biology). 参考文献を見る
  3. Kronfeld I, Kazimirsky G, Lorenzo PS, Garfield SH, Blumberg PM, Brodie C. Phosphorylation of protein kinase Cdelta on distinct tyrosine residues regulates specific cellular functions. J Biol Chem. 2000; 275(45):35491-35498. (Biology: Western blot). 参考文献を見る
  4. Labow BI, Souba WW, Abcouwer SF. Glutamine synthetase expression in muscle is regulated by transcriptional and posttranscriptional mechanisms. Am J Physiol. 1999; 276(6):E1136-E1145. (Biology: Western blot). 参考文献を見る
  5. Vardimon L, Fox LE, Cohen-Kupiec R, Degenstein L, Moscona AA. Expression of v-src in embryonic neural retina alters cell adhesion, inhibits histogenesis, and prevents induction of glutamine synthetase. Mol Cell Biol. 1991; 11(10):5275-5284. (Biology). 参考文献を見る
すべて表示する (5) 表示項目を減らす
610517 Rev. 2

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