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Purified Mouse Anti-Gephyrin
Purified Mouse Anti-Gephyrin

Western blot analysis of Gephyrin on rat brain lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Gephyrin.

Purified Mouse Anti-Gephyrin

Rat cerebellum, formalin-fixed paraffin embedded tissue, with citrate pre-treatment, 20X.

Western blot analysis of Gephyrin on rat brain lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Gephyrin.

Rat cerebellum, formalin-fixed paraffin embedded tissue, with citrate pre-treatment, 20X.

製品詳細
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BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse, Chicken (Tested in Development)
Mouse IgG1
Rat Gephyrin aa. 569-726
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
93 kDa
250 µg/ml
AB_397929
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610585 Rev. 1
抗体の詳細
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45/Gephyrin

The sub-membraneous region of the postsynaptic neuron is an intricate network of cytoskeletal elements generally known as the postsynaptic density. It is thought that this elaborate cytoskeletal region is critical for receptor targeting, clustering, and efficient signal input. Gephyrin, a 93 kDa protein, was identified as a result of its ability to bind to polymerized tubulin (microtubules). Although expressed in all tissues, gephyrin mRNA is found most abundantly in brain. Alternative splicing produces at least five different mRNAs. Gephyrin colocalizes and interacts with the glycine receptor at the postsynaptic density. It is possible that Gephyrin acts as an anchor between the glycine receptor and microtubules.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610585 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610585 Rev.1
引用&参考文献
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開発者向け参考資料 (4)

  1. Feng G, Tintrup H, Kirsch J, et al. Dual requirement for gephyrin in glycine receptor clustering and molybdoenzyme activity. Science. 1998; 282(5392):1321-1324. (Clone-specific: Western blot). 参考文献を見る
  2. Kneussel M, Haverkamp S, Fuhrmann JC, et al. The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse. Proc Natl Acad Sci U S A. 2000; 97(15):8594-8599. (Clone-specific: Immunofluorescence, Western blot). 参考文献を見る
  3. Meyer G, Kirsch J, Betz H, Langosch D. Identification of a gephyrin binding motif on the glycine receptor beta subunit. Neuron. 1995; 15(3):563-572. (Biology). 参考文献を見る
  4. Prior P, Schmitt B, Grenningloh G, et al. Primary structure and alternative splice variants of gephyrin, a putative glycine receptor-tubulin linker protein. Neuron. 1992; 8(6):1161-1170. (Biology). 参考文献を見る
すべて表示する (4) 表示項目を減らす
610585 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.