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Purified Mouse Anti-Flotillin-1
製品詳細
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BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse, Chicken (Tested in Development)
Mouse IgG1
Mouse Flotillin aa. 312-428
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
48 kDa
250 µg/ml
AB_398139
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610821 Rev. 1
抗体の詳細
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18/Flotillin-1

Caveolae are specialized membrane invaginations of 50-100 nm present in all cells, but abundant in endothelium, muscle cells, and adipocytes. These plasma membrane microdomains function in transcytosis of macromolecules, and are the sites of potocytosis, where small molecules are concentrated and transferred inside the cells by glycosylphosphatidylinositol (GPI)-linked receptors. Caveolin, a 22kDa protein and a well known marker for these plasma membrane microdomains, plays a structural role in these specializations. Flotillin-1 was isolated from the Triton X-100 insoluble buoyant fraction, characteristic of caveolae. Although the mRNA expression of both Flotillin-1 and Caveolin is very similar, Caveolin is undetectable in brain, while Flotillin-1 is very abundant. Flotillin-1 is a close homolog of the Epidermal Surface Antigen (ESA/Flotillin-2), which also colocalizes in the caveolae. Thus, Flotillin-1 and its relative ESA/Flotillin-2 are now incorporated into the expanding list of proteins co-localized at the caveolae which includes PKCα, Ras, Rap Src-like kinases, Gαßγ,and GPI-linked receptors.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610821 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610821 Rev.1
引用&参考文献
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開発者向け参考資料 (5)

  1. Bickel PE, Scherer PE, Schnitzer JE, Oh P, Lisanti MP, Lodish HF. Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins. J Biol Chem. 1997; 272(21):13793-13802. (Biology). 参考文献を見る
  2. Cabin DE, Shimazu K, Murphy D, et al. Synaptic vesicle depletion correlates with attenuated synaptic responses to prolonged repetitive stimulation in mice lacking alpha-synuclein. J Neurosci. 2002; 22(20):8797-8807. (Clone-specific: Western blot). 参考文献を見る
  3. Fallon L, Moreau F, Croft BG, Labib N, Gu WJ, Fon EA. Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain. J Biol Chem. 2002; 277(1):486-491. (Clone-specific: Western blot). 参考文献を見る
  4. Morrow IC, Rea S, Martin S, et al. Flotillin-1/reggie-2 traffics to surface raft domains via a novel golgi-independent pathway. Identification of a novel membrane targeting domain and a role for palmitoylation. J Biol Chem. 2002; 277(50):48834-48841. (Clone-specific: Immunofluorescence). 参考文献を見る
  5. Rybin VO, Xu X, Lisanti MP, Steinberg SF. Differential targeting of beta -adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signaling pathway. J Biol Chem. 2000; 275(52):41447-41457. (Clone-specific: Electron microscopy, Western blot). 参考文献を見る
すべて表示する (5) 表示項目を減らす
610821 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.