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Purified Mouse Anti-Ezrin
Purified Mouse Anti-Ezrin

Western blot analysis of Ezrin on MDCK lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Ezrin.

Purified Mouse Anti-Ezrin

Immunofluorescent staining of HeLa cells with anti-Ezrin antibody.

Western blot analysis of Ezrin on MDCK lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Ezrin.

Immunofluorescent staining of HeLa cells with anti-Ezrin antibody.

製品詳細
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BD Transduction Laboratories™
Dog (QC Testing), Human (Tested in Development)
Mouse IgG1
Human Ezrin aa. 391-515
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
80 kDa
250 µg/ml
AB_397940
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610603 Rev. 1
抗体の詳細
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18/Ezrin

First described as an 80kDa protein concentrated in the apical cytoskeletal region of intestinal brush border cells, ezrin is now recognized as a major substrate of protein tyrosine kinases, such as the epidermal growth factor (EGF) tyrosine kinase. Ezrin is expressed at high levels in intestine, kidney, and placenta. In placenta, ezrin is present as monomers and non-covalent oligomers in tight association with actin microfilaments. In the human epidermoid carcinoma cell line A431, microvilli-like structures appear within 30 seconds after the addition of EGF. These structures give way to membrane ruffles after 2-5 minutes, followed by cell rounding after 10-20 minutes. At the same time, ezrin is recruited into these structures and oligomers are formed following its tyrosine phosphorylation. It is thought that tyrosine phosphorylation triggers the formation of ezrin oligomers.

610603 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610603 Rev.1
引用&参考文献
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開発者向け参考資料 (5)

  1. Anastasiadis PZ, Moon SY, Thoreson MA, et al. Inhibition of RhoA by p120 catenin. Nat Cell Biol. 2000; 2(9):637-644. (Clone-specific: Immunofluorescence). 参考文献を見る
  2. Berryman M, Gary R, Bretscher A. Ezrin oligomers are major cytoskeletal components of placental microvilli: a proposal for their involvement in cortical morphogenesis. J Cell Biol. 1995; 131(5):1231-1242. (Biology). 参考文献を見る
  3. Defacque H, Egeberg M, Habermann A, et al. Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes. EMBO J. 2000; 19(2):199-212. (Clone-specific: Western blot). 参考文献を見る
  4. Mohler PJ, Kreda SM, Boucher RC, Sudol M, Stutts MJ, Milgram SL. Yes-associated protein 65 localizes p62(c-Yes) to the apical compartment of airway epithelia by association with EBP50. J Cell Biol. 1999; 147(4):879-890. (Clone-specific: Immunofluorescence). 参考文献を見る
  5. Perez OD, Kinoshita S, Hitoshi Y, et al. Activation of the PKB/AKT pathway by ICAM-2. Immunity. 2002; 16(1):51-65. (Clone-specific: Immunoprecipitation, Western blot). 参考文献を見る
すべて表示する (5) 表示項目を減らす
610603 Rev. 1

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