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Purified Mouse Anti-EphA4/Sek
Purified Mouse Anti-EphA4/Sek
Western blot analysis of EphA4 on rat brain lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of EphA4.
Purified Mouse Anti-EphA4/Sek

Immunofluorescence staining of PC12 cells

Western blot analysis of EphA4 on rat brain lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of EphA4.

Immunofluorescence staining of PC12 cells

製品詳細
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BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse, Dog, Chicken (Tested in Development)
Mouse IgG1
Mouse Sek aa. 279-472
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
110-130 kDa
250 µg/ml
AB_397843
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610471 Rev. 1
抗体の詳細
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35/EphA4

EphA4/Sek is a glycosylated protein tyrosine kinase of 986 amino acids and an apparent 110-130kDa molecular weight (molecular weight depends on glycocylation level of protein). EphA4 belongs to the Eph subfamily of receptor tyrosine kinases and like many others, it has an amino-terminal ligand-binding domain exposed to the extracellar matrix. EphA4 is predominantly expressed during development (this is common in the Eph family) and because of its mRNA localization, it has been proposed that it may play a role during segmentation of the hindbrain and mesoderm. Tyrosine 602 is a major autophosphorylation site of EphA4 and appears to be the docking site for the intracellular tyrosine kinase fyn.

610471 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610471 Rev.1
引用&参考文献
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Development References (3)

  1. Becker N, Gilardi-Hebenstreit P, Seitanidou T, Wilkinson D, Charnay P. Characterisation of the Sek-1 receptor tyrosine kinase. FEBS Lett. 1995; 368(2):353-357. (Biology). View Reference
  2. Ellis C, Kasmi F, Ganju P, Walls E, Panayotou G, Reith AD. A juxtamembrane autophosphorylation site in the Eph family receptor tyrosine kinase, Sek, mediates high affinity interaction with p59fyn. Oncogene. 1996; 12(8):1727-1736. (Biology). View Reference
  3. Gilardi-Hebenstreit P, Nieto MA, Frain M. An Eph-related receptor protein tyrosine kinase gene segmentally expressed in the developing mouse hindbrain. Oncogene. 1992; 7(12):2499-2506. (Biology). View Reference
610471 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.